Table 3. Performance of microbial standard DNA in qPCR reactions.

16S	Standardtype	Standard curvea	R2	Efficiency (%)b
Bacteriac	Amplicon	y = −3.74±0.03x +37.41±0.21	0.999	84.9
	Linear	y = −3.75±0.02x +38.05±0.14	0.999	84.7
	Nicked circles	y = −3.61±0.04x +37.49±0.20	0.999	87.3
	Supercoiled	y = −3.63±0.03x +38.26±0.15	0.999	88.7
Archaead	Amplicon	y = −3.64±0.01x +36.99±0.07	0.999	88.4
	Linear	y = −3.62±0.06x +37.22±0.32	0.999	89.0
	Nicked circles	y = −3.48±0.06x +36.62±0.31	0.999	93.7
	Supercoiled	y = −3.56±0.02x +37.15±0.13	0.999	91.0

^aLinear regression between Ct (y-intercept) and log₁₀ starting copy number (x, i.e. slope).

^bEfficiency (%) calculated: E = (10^1/slope−1)×100.

^cBacteria: T. lienii.

^dArchaea: A. fulgidus.