Figure 2. Recombinant mouse Slit2 (mSlit2) inhibits the extension of neurite-like processes in CAD cells.

(A, B) Differentiated CAD cells were plated at low density and cultured for 72 hr in the absence or presence of recombinant mSlit2 (25 nM) and/or recombinant rRobo1 (56 nM), as indicated. CAD cells were fixed and immunostained for tubulin. Representative images are shown in (A), and quantification of the lengths of neurite-like processes is shown in (B). Bar, 200 µm. *** p<0.001.