Figure 5. Time-course of process extension in the presence or absence of mSlit2.

(A, B) Differentiated CAD cells were plated at low density and cultured for the indicated period of time in the absence or presence of recombinant mSlit2 (25 nM). Cells were fixed and stained for tubulin at three different time points (24 hr, 48 hr, 72 hr). Representative images are shown in (A), and quantification of the lengths of neurite-like processes is shown in (B). Bar, 200 µm.