Figure 2. Realtime RT-PCR for relative mRNA level (2^−ΔΔCt) of NP markers.

(A) Basp1; (B) Ncdn; (C) Nrp-1; (D) Brachyury T; (E) CD155; (F) CD221 in rat IVD tissues at different ages (1 m, 12 m and 21 m). All values of fold-difference were normalized to AF tissue (1 m) for comparison between different tissue regions and ages. m: month. * p<0.05, ** p<0.01, two-factor ANOVA. (TIF)