Table 3.
PCR/Electron spray ionization-time-of-flight-mass spectrometry (PCR/ESI-TOF-MS) versus traditional clinical microbiology (TCM) for detection of most frequently recovered potentially clinically relevant pathogens, plus coagulase negative staphylococci (CNS), contaminating healthcare workers and personal protective equipment
| |
PCR/ESI-TOF-MS (n = 77) |
TCM (n = 23) |
||||||
|---|---|---|---|---|---|---|---|---|
| Organism | Hands pre-care | Gloves | Gowns | Hands post-care | Hands pre-care | Gloves | Gowns | Hands post -care |
| CNS |
12 |
6 |
8 |
12 |
4 |
4 |
3 |
3 |
|
Staphylococcus aureus |
|
3 |
3 |
1 |
|
3 |
1 |
1 |
| Streptococci* |
9 |
4 |
3 |
1 |
|
1 |
|
|
|
Acinetobacter spp.** |
|
3 |
1 |
|
|
1 |
|
|
|
Enterobacter spp.*** |
|
1 |
|
|
|
2 |
|
|
|
Escherichia coli |
|
1 |
1 |
|
|
|
|
|
|
Klebsiella pneumoniae |
1 |
3 |
1 |
1 |
|
|
|
|
|
Pseudomonas spp.**** |
1 |
|
|
1 |
|
|
|
|
| Candida spp.***** | 2 | |||||||
*TCM: 1 Group D Streptococcus; PCR/ESI-TOF-MS: 7 S. agalactiae, 10 viridans group streptococci.
**TCM: 1 A. baumannii; PCR/EIS-MS: 2 A. baumannii, 2 A. calcoaceticus.
***TCM: 2 Enterobacter aerogenes; PCR/ESI-TOF-MS: 1 E. aerogenes.
****PCR/ESI-TOF-MS: 1 Pseudomonas fluorescens, 1 Pseudomonas mendocina.
*****PCR/ESI-TOF-MS: 1 C. albicans, 1 Candida glabrata.