Table 4.
PCR/Electron spray ionization-time-of-flight-mass spectrometry (PCR/ESI-TOF-MS) versus traditional clinical microbiology (TCM) for detection of most frequently recovered potentially clinically relevant organisms, plus coagulase negative staphylococci (CNS), contaminating high-use surfaces
|
Bacteria |
PCR/ESI-TOF-MS (# isolates) n = 305 |
TCM (# isolates) n = 89 |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Bedrail | Door handle | Faucet | IV pump | Keyboard | Mouse | Shears | Bedrail | Door handle | Faucet | IV pump | Keyboard | Mouse | Shears | |
| CNS |
24 |
31 |
28 |
23 |
30 |
22 |
19 |
11 |
6 |
14 |
9 |
16 |
9 |
7 |
| Enterococci* |
|
2 |
|
|
|
|
1 |
2 |
1 |
|
1 |
|
|
|
|
S. aureus |
4 |
|
1 |
4 |
2 |
3 |
|
3 |
|
1 |
1 |
1 |
2 |
|
| Streptococci** |
7 |
8 |
12 |
12 |
17 |
7 |
5 |
|
|
|
|
|
|
1 |
|
Acinetobacter spp.*** |
2 |
1 |
2 |
6 |
|
|
|
|
1 |
|
1 |
|
|
|
|
Enterobacter spp.**** |
3 |
1 |
1 |
1 |
|
|
|
1 |
1 |
|
|
|
|
|
|
E. coli |
2 |
1 |
|
1 |
|
|
|
|
|
|
|
|
|
|
|
K. pneumoniae |
7 |
|
1 |
3 |
1 |
3 |
|
|
|
|
|
|
|
|
|
Pseudomonas spp.***** |
4 |
|
2 |
|
1 |
|
|
|
|
|
|
|
|
|
| Candida spp.****** | 7 | 3 | 5 | 3 | 1 | 2 | ||||||||
*TCM: “Enterococcus spp.”; PCR/ESI-TOF-MS: 2 Enterococcus faecalis, 1 Enterococcus faecium.
**TCM: 1 viridans group streptococci; PCR/ESI-TOF-MS: 1 Group D Streptococcus, 24 S. agalactiae, 8 Streptococcus pneumoniae, 1 S. pyogenes, 1 “Streptococcus spp.”, 33 viridans group streptococci.
***TCM: 2 A. baumannii; PCR/ESI-TOF-MS: 10 A. baumannii, 1 “Acinetobacter spp.”
****TCM: 2 Enterobacter cloacae; PCR/ESI-TOF-MS: 4 E. cloacae; 2 Enterobacter sakazakii.
*****PCR/ESI-TOF-MS: 1 P. aeruginosa, 2 Pseudomonas entomophila/putida, 3 P. fluorescens, 1 “Pseudomonas spp.”
******PCR/ESI-TOF-MS: 10 C. albicans, 4 C. glabrata, 1 Candida kefyr, 1 Candida kruseii, 1 Candida parapsilosis, 1 Candida rugosa, 3 Candida tropicalis.