Table 1. Primer sequences and cycling conditions used for amplification.
Name | 5′ Primer sequence | DNA region | Fragment size |
IFb-16128 | GGTACCATAAATACTTGACCACCT | HVI | 221 bp |
IR-16348 | GACTGTAATGTGCTATGTACGGTAAA | ||
IIFa-45 | ATGCATTTGGTATTTTCGTCTG | HVII | 243 bp |
IIR-287 | TTGTTATGATGTCTGTGTGGAAAG | ||
15971 | TTAACTCCACCATTAGCACC | HVI | 440 bp |
16410 | GAGGATGGTGGTCAAGGGAC | ||
15971 | TTAACTCCACCATTAGCACC | HVI | 616 bp |
R17 | CCC GTG AGT GGT TAA TAG GGT | ||
Amelogenin F | CCCTGGGCTCTGTAAAGAATAGT | Chr X and Y | 106 bp (XX) |
Amelogenin R | ACTAGAGCTTAAACTGGGAAGCTG | 112 bp (XY) |