Table 6.
NO2-production of J774 cells: Mouse macrophages untreated, treated with RJI-C, TB-KK or the MIX, stimulated with LPS, stimulated with LPS and treated with RJI-C, TB-KK or the MIX
| Treatment |
Time of incubation (h) |
||
|---|---|---|---|
| 24 | 48 | 72 | |
| No treatment |
0.25 ± 0.04 |
0.69 ± 0.02 |
0.92 ± 0.2 |
| RJI-C (15 μg/ml) (12 μM) |
0.42 ± 0.03 |
0.75 ± 0.01 |
1.02 ± 0.3 |
| TB-KK (15 μg/ml) (6.5 μM) |
0.82 ± 0.05 |
1.25 ± 0.2 |
1.34 ± 0.2 |
| MIX (RJI-C 9 μg/ml + TB-KK 6 μg/ml) (RJI-C 7.3 μM + TB-KK 2.6 μM) |
0.72 ± 0.3 |
0.85 ± 0.3 |
1.06 ± 0.2 |
| LPS (10 μg/ml) |
2.93 ± 0.2 |
10.96 ± 0.4 |
12.16 ± 0.5 |
| LPS + RJI-C (15 μg/ml) (12 μM) |
2.85 ± 0.3 |
8.42 ± 0.1 |
10.21 ± 0.2 |
| LPS + TB-KK (15 μg/ml) (6.5 μM) |
3.12 ± 0.6 |
9.75 ± 0.1 |
11.45 ± 0.2 |
| LPS + MIX (RJI-C 9 μg/ml + TB-KK 6 μg/ml) (RJI-C 7.3 μM + TB-KK 2.6 μM) | 2.63 ± 0.4 | 7.25 ± 0.3 | 8.26 ± 0.1 |
Data are expressed as micromoles of NO2- for 106 input cells, and are means ± standard deviation of three different experiments each performed in triplicate.