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Three harvest methods exemplarily shown with C. glutamicum producing bpr-Cutinase.A: Synchronous harvest of all cultivation wells in the stationary phase (“over night cultivation”). B: Triggered harvest of total reaction volume 4 h after reaching 4 g.L-1 CDW (BS = 100 a.u.) in early stationary phase. C: Triggered induction at 4 g.L-1 CDW (BS = 100 a.u.) and triggered harvest 4 h later in early stationary phase.
