Table 1.
Influence of three harvest methods on the lipolytic activity (EAspec) fused with three different signal peptides (bpr, amyE, ywmC)
| A: EAspec[kU.g-1] “Over night cultivation” | B: EAspec[kU.g-1] Triggered harvest | C: EAspec[kU.g-1] Triggered induction + harvest | |
|---|---|---|---|
| bpr |
10.11 ± 2.98 |
11.84 ± 0.96 |
13.34 ± 1.25 |
| ywm |
3.61 ± 1.50 |
5.31 ± 0.60 |
4.40 ± 0.54 |
| amyE |
1.22 ± 0.64 |
1.62 ± 0.33 |
1.49 ± 0.24 |
| Mean relative error |
41% |
13% |
13% |
| Duration of stationary phase | 6.0 h ± 1.7 h | 0.9 h ± 0.4 h | 1.6 h ± 0.3 h |
Error indicate standard deviation of 9 to 12 fold biological replicates (independent clones). Depending on the sampling method, the mean duration of stationary phase is different. Backscatter data of all processes are provided as supplemental data.