Figure 3.

Inhibition of thiol-disulfide exchange suppresses HIV-1 infection of primary human MDM. After preincubation for 30 minutes. with various concentrations of DTNB, anti-PDI or anti-Trx mAbs, MDM were infected (in duplicate, in the presence of the inhibitors) for 4 h with the HIV-1 strains indicated, washed twice, and then maintained in MØ medium without the reagents. Cell culture supernatants were harvested and replenished (80% v/v) every three days, and harvested supernatants were stored at −80°C before being analyzed for reverse transcriptase (RT) activity.