Figure 2. Determination of mVCAM-1 mRNA and protein expression by quantitative real-time PCR and western blot.

A. mVCAM-1 expression after adiponectin (0.1, 1, 5, 10 µg/ml) treatment in ATDC-5 cell line, during 24 hours. B. mVCAM-1 expression after IL-1β (10 ng/ml), adiponectin (10 µg/ml) and the combination of IL-1β (10 ng/ml) plus adiponectin (10 µg/ml) challenge in ATDC-5 cell line, during 24 hours.