Skip to main content
. 2012 Dec 19;7(12):e52567. doi: 10.1371/journal.pone.0052567

Figure 2. hCG-β localization in human retina.

Figure 2

Vimentin labeled Muller glial cell of the retina mainly in the GCL and IPL (A, C, D, F). Müller glia cell processes are labeled with vimentin (A, D) and hCG-β (B, E) between the ganglion nucleus that are labeled in blue in the GCL (C, F). Arrow (A–F) depicts glia cell endfeet that is double labeled with hCG-β (C, F). Diffuse staining against hCG-β suggests a secretion of hCG in the retina (B, E). hCG-β localization in human RPE. Primary RPE cells kept in culture were stained with phalloidin (G) and hCG-β (H), Merged image (I) revealed the expression of this gonadotropin in the RPE cells. Scale bar is 30 µm for A–C 15 µm for D–F and 10 µm for G–I. A, D: Mouse monoclonal anti vimentin (Abcam ref# ab28028) use 1/100 in PBS, 0.1% BSA, 0.01% triton x100; B, E, H, I: Rabbit polyclonal anti hCG-β (Abcam ref# ab9376) use 1/100 in PBS, 0.1% BSA, 0.01% triton x100; G, I : Phalloidin-488 (Millipore ref# A12379) use at 1/50 in PBS; C, F, I : Merged image, with blue channel corresponding to DAPI nucleus staining used at 1/1000. ONL = Outer nuclear layer, OPL = outer plexiform layer, INL = Inner nuclear layer, IPL = Inner plexiform layer, GCL = Ganglion cell layer.