Table 1. Properties of the acoustic unit.

Transducer	planar - 50 mm in diameter
Central frequency (MHz)	1.0
Device - indicated intensity (W/cm2) *	0.1	0.2	0.3	0.4	0.5
ISATA at 10% DC (W/cm2) †	0.048	0.072§	0.081§	0.092§	0.105§
Output pressure (MPa) ‡	0.061	0.105	0.132	0.144	0.146
Operation modes	Continuous wave mode – Pulsed wave mode
Duty Cycle (DC, %)	10, 20, 30, 50 & 100
Pulse Repetition Frequency (PRF, Hz)	100

^*The intensity ranges from 0.1–2 W/cm² at an increment of 0.1 W/cm².

^†The output intensity measured by an ultrasound power meter (UPM-DT-10E, Ohmic Instrument Co., Easton, MD) from the transducer surface and divided by the effective surface area of the transducer. Attenuation due to the culture dish was practically negligible (<10%).

^‡The output pressure calculated from the equation I = P²/ρc, where (I) is the intensity [W/m²], (P) is the acoustic pressure, (ρ) is the density of the medium and (c) is the speed of sound in the medium.

^§The intensity used in sonication experiments.