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. 2012 Dec 19;7(12):e51748. doi: 10.1371/journal.pone.0051748

Figure 6. The MRM measurement of serum concentrations of A1BG, LRG1 and their association with NSCLC.

Figure 6

(A–B) MRM Intensities of selected reference peptides of A1BG and LRG1 both showed good linear correlation with on-column abundance. The x-axis represents base-3 logarithm of ratios of spiked light and heavy isotopic peptides, with the y-axis corresponding to the observed peak area ratios in base-3 logarithmic scale. Red triangles suggest the limit of linear quantification (LOQ) of each peptide. Note that these two peptides were used to report the absolute concentration, with comparison to another two less-optimal peptides shown in Figure S4. (C–D) The chromatography peaks of the best transitions of two peptides for A1BG and LRG1. Note that the Signal-to-Noise ratios are sufficient for quantification. (E) The distribution of serum levels of A1BG and LRG1 between cancer and control groups. (F) ROC analysis suggested over-expressed A1BG and LRG1 were associated with NSCLC and their combined panel could provide the better discriminative performance than each of the protein.