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. 2012 Dec 19;7(12):e51757. doi: 10.1371/journal.pone.0051757

Table 1. Forward (F) and Reverse (R) primers for the amplification of 16S rRNA (16S), ITS, rnpB and rpoB fragments for GS-FLX DNA sequencing.

Loci Primer sequence (5′ to 3′) Approxiate amplicon length (bp) including primers
16S-F GAGGTTGATCATGGCTCAG 238–248
16S-R ACAACGCAGGTCCATCT
ITS-F AGGTAGCCGTATCGGAAGGT 231–250
ITS-R YACAGCGTTTTCGGTTTATTT
rnpB-F GTGCAATTTTTGGATAATCG 248–254
rnpB-R TGGGTTGCTAGCTTGAGG
rpoB-F CTGAAGAACGTCTCTTGCAC 245
rpoB-R CGAGAGACAACCCCTTTRTT