Table 1. Forward (F) and Reverse (R) primers for the amplification of 16S rRNA (16S), ITS, rnpB and rpoB fragments for GS-FLX DNA sequencing.
Loci | Primer sequence (5′ to 3′) | Approxiate amplicon length (bp) including primers |
16S-F | GAGGTTGATCATGGCTCAG | 238–248 |
16S-R | ACAACGCAGGTCCATCT | |
ITS-F | AGGTAGCCGTATCGGAAGGT | 231–250 |
ITS-R | YACAGCGTTTTCGGTTTATTT | |
rnpB-F | GTGCAATTTTTGGATAATCG | 248–254 |
rnpB-R | TGGGTTGCTAGCTTGAGG | |
rpoB-F | CTGAAGAACGTCTCTTGCAC | 245 |
rpoB-R | CGAGAGACAACCCCTTTRTT |