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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Shock. 2013 Jan;39(1):3–10. doi: 10.1097/SHK.0b013e3182764fe8

Fig. 7. Effect of Wnt agonist on induction of apoptosis after hepatic I/R.

Fig. 7

Liver tissues were harvested 24 h after reperfusion. (A-C) Liver sections were subjected to TUNEL assay to detect DNA fragmentation. Representative images for sham (A), vehicle (B), and Wnt agonist-treated (C, WntAg) groups. Green, TUNEL positive and red, propidium iodide staining for nucleus. (D) A graphical representation of TUNEL positive staining cells averaged over 10 microscopic fields/animal. (E) Liver tissue caspase-3 activity was measured by a fluorometric assay. Data presented as means ± SE (n=4-6/group) and compared by one-way ANOVA and SNK method;*P > 0.05 vs. Sham; #P > 0.05 vs. Vehicle.