Table 3.
Average cycle thresholds ± STDEV of GeneDisc assays using BPW for enrichment of ground beef samples.
| Serogroup-straina | stx1 | stx2 | eae | ehxA | O157b | O groupb |
|---|---|---|---|---|---|---|
| O26 | 22.7 ± 3.2 | – | 24.8 ± 4.1 | 23.2 ± 3.2 | – | 22.5 ± 3.1 |
| O45 | 20.9 ± 3.7 | – | 23.6 ± 4.3 | 21.6 ± 3.7 | – | 22.6 ± 3.4 |
| O91 | – | 22.7 ± 3.7 | – | 22.0 ± 4.5 | – | 22.4 ± 3.5 |
| O103 | 22.2 ± 3.1 | – | 23.8 ± 3.2 | 22.4 ± 3.2 | – | 23.3 ± 2.7 |
| O111 | ||||||
| 00-4748 | 22.8 ± 4.6 | 23.0 ± 6.0 | 23.2 ± 2.6 | – | – | 21.9 ± 4.2 |
| 98-8338 | 21.7 | – | 22.7 | 21.7 | – | 20.4 |
| 01387 | 21.1 | – | 22.2 | 20.7 | – | 20.3 |
| SJ14 | 20.0 | 20.8 | 22.0 | 20.6 | – | 20.3 |
| SJ15 | 21.4 | 18.6 | 23.2 | 19.0 | – | 21.2 |
| E05 | 20.0 | – | 21.2 | 19.4 | – | 19.4 |
| O113 | – | 20.7 ± 2.8 | – | 21.1 ± 3.2 | – | 21.2 ± 3.0 |
| O121 | – | 24.6 ± 6.7 | 26.3 ± 5.9 | 24.2 ± 5.9 | – | 24.6 ± 4.4 |
| O145 | 22.9 ± 3.7 | – | 25.2 ± 4.7 | 23.5 ± 3.9 | – | 25.3 ± 3.7 |
| O157 | 18.8 ± 2.3 | 19.2 ± 2.3 | 19.6 ± 0.9 | 20.0 ± 1.8 | 19.1 ± 1.0 | 20.9 ± 3.3 |
a
Results are averages from at least three separate experiments except for O111 strains 98-8338, O1387, SJ14, SJ15, and E05 for which only one experiment was performed.
b
Results in the column labeled O157 represent data from the STEC and E. coli O157 GeneDisc that targeted virulence genes, as well as O157. The column labeled O group shows data from the GeneDisc 9 EHEC Identification that targeted the 9 O serogroups.