Fig. 3.

HUVEC spheroid sprouting inhibition with DARPins. HUVEC spheroids were incubated with VEGF-A, mixed with either PBS, or test substances. Test substances were DARPins #2 and #4, and ranibizumab (2, 4, and L (Lucentis), respectively). a Average accumulated total sprout length per spheroid. All groups (L, 2 and 4) are statistically different from PBS controls using ANOVA analysis and Tukey Multiple Comparison post hoc test (p < 0.001). b Representative pictures of sprouting spheroids. The isotype control DARPin I2 showed equivalent results as the PBS control