Fig. 3.

Effect of Cyperi rhizoma (CR) and Corydalis tuber (CT) on chronic constriction injury (CCI)-induced phosphorylation of NMDA receptor NR1 subunit (pNR1) in induction phase. The number of pNR-1 positive neurons was significantly increased by CCI surgery (B) as compared with Sham group (A). Treatment of CR or CT (30 mg/kg) in the induction phase significantly reduced the number of pNR1-immunoreactive neurons both in superficial dorsal horn (SDH) and nucleus proprius (NP) as compared with that of control group (B). However, there is no statistically significant change in neck of dorsal horn (NECK) area. The pNR1 immunoreactivity was observed in Sham (A), control (B), CR (C) and CT (D)-treated groups. Panel (E) shows a diagram depicting the location of the different spinal cord regions analyzed in this study. Results of each group are represented in panel (F). Inserted panels show a magnified image of rectangles in panel A, B, C, and D, respectively. The black arrow heads indicate representative pNR1 neurons. The number of animals is 3 in each group. Scale bar=200 µm. ^*p<0.05 and ^**p<0.01 compared to control.