Table 1.

Structural parameters of intact SPMV, SPMVTL and SPMVPL

^a)SPMV was treated±4 mM TNBS, pH 8.5, at 4℃ for 80 min (I'/I). SPMVTL and SPMVPL were treated±0.5 mM TNBS, pH 8.5, at 4℃ for 20 min (I'/I). Py-3-Py was incorporated and fluorescence measurements were performed at 37℃ (pH 7.4); ^b)SPMV was treated±2 mM TNBS, pH 8.5, at 4℃ for 40 min (anisotropy). SPMVTL and SPMVPL were treated±0.5 mM TNBS, pH 8.5, at 4℃ for 20 min (anisotropy). DPH was incorporated and fluorescence measurements were performed at 37℃ (pH 7.4).

Values from untreated membranes represent inner+outer monolayer; Values from TNBS treated membranes represent the inner monolayer; Values for the outer monolayer were calculated as described in Materials and Methods. Values are represented as the mean±SEM of 5 determinations.