Fig. 1.

Induction of iNOS expression by GDB plus IFN-γ. (A) GDB was isolated as described in "Methods". Isolated GDB was diluted 2- and 4- folds and analyzed by SDS-PAGE. (B) RAW264.7 cells were treated with GDB (25 µg/ml) and IFN-γ (10 ng/ml) for 24 h. Morphological change and iNOS expression were analyzed using immunofluorescence staining (upper column; with staining, lower column; without staining) and inverted microscope. (C) RAW264.7 cells were treated with GDB (1, 10, and 25 µg/ml) in the presence or absence of IFN-γ (10 ng/ml) for 8 h. Total RNA was then isolated and analyzed for iNOS using RT-PCR. (D) RAW264.7 cells were treated with GDB (1, 10, and 25 µg/ml) in the presence or absence of IFN-γ for 24 h. Cell lysates were then prepared and analyzed for the iNOS using Western blot analysis. One representative of three experiments is shown.