Fig. 3.

Activation of p38 by GDB plus IFN-γ. (A) RAW264.7 cells were treated with GDB (1, 10, and 25 µg/ml) in the presence or absence of IFN-γ (10 ng/ml) for 30 min. Cell lysates were prepared and subjected to immunoblot assay using antibodies against phospho- and nonphospho-p38, ERK1/2 and SAPK/JNK proteins. (B) The density of p38 was analyzed by Image J program. β-actin was used as a control. One representative of three experiments is shown. ^*Denotes significant differences (p<0.05) from the control group.