Fig. 3.

Effects of OxPAPC on secretion and gene transcription of CCL2 and IL-1β. (A) THP-1 cells were stimulated for 9 h with or without FSL-1 (100 ng/ml) after treatment for 1 h with OxPAPC (30 µg/ml). The amounts of CCL2 and IL-1β released into the medium were measured by ELISA. ^***p<0.001 vs. control. ^##p <0.01 vs. FSL-1. ^###p<0.001 vs. FSL-1. (B) Transcripts of CCL2 and IL-1β genes were amplified by RT-PCR after the treatment. ^**p<0.01 vs. control. ^###p<0.001 vs. FSL-1.