Fig. 5.

Effects of MAPKs inhibitors on secretion and gene transcription of CCL2 and IL-1β. (A) THP-1 cells were exposed to FSL-1 for the indicated time periods; an equal amount of protein was then analyzed by western blotting using antibodies against phosphorylated and unphosphorylated forms of ERK, p38 MAPK, and JNK. (B) THP-1 cells were stimulated for 9 h with or without FSL-1 (100 ng/ml) after pretreatment for 1 h with the indicated MAPKs inhibitors (10 µM each). The amounts of CCL2 and IL-1β released into the medium were measured by ELISA. ^***p<0.001 vs. control. ^##p<0.01 vs. FSL-1. ^###p<0.001 vs. FSL-1. (C) Transcripts of CCL2 and IL-1β genes were amplified by RT-PCR. ^***p<0.001 vs. control. ^#p<0.05 vs. FSL-1. ^##p<0.01 vs. FSL-1. ^###p<0.001 vs. FSL-1.