Fig. 6.

Effects of PKC inhibitors on secretion and gene transcription of CCL2 and IL-1β. (A) THP-1 cells were exposed to FSL-1 for the indicated time periods; PKC activity was then determined using the PKC kinase activity assay kit. Data are expressed as mean±SD. ^***p<0.01 vs. 0 min. (B) THP-1 cells were stimulated for 9 h with or without FSL-1 (100 ng/ml) after pretreatment with RO318220 (1 µM) and GF109203X (3 µM). The amounts of CCL2 and IL-1β released into the medium were measured by ELISA. ^***p<0.001 vs. control. ^##p<0.01 vs. FSL-1. ^###p<0.001 vs. FSL-1. (C) Transcripts of CCL2 and IL-1β genes were amplified by RT-PCR after the treatment. ^***p<0.001 vs. control. ^###p<0.001 vs. FSL-1.