Figure 1.

Gadd45γ Is Expressed in Somatic Cells of the Developing XX and XY Gonad with a Spatiotemporal Profile Reminiscent of Sry

(A–C) WMISH in the developing XX (A) and XY (B) wild-type gonads at 11.5 dpc (19 ts). Expression is restricted to the body of the gonad and not detected in the coelomic epithelium (C, arrowhead). Dotted line in (B) indicates the position of the section shown in (C). Scale bar in (C) is 100 μm.

(D and E) Two-color WMISH with Oct4 (red) and Gadd45γ (blue).

(F–I) Oct4 is not detected in W/W homozygous gonads at 11.5 dpc (F and G), in contrast to Gadd45γ expression (H and I).

(J–L) Gadd45γ is first detected at 9 ts, in the central portion of the gonad (J and K); white arrowheads mark anterior and posterior limits of the gonad in (J–L). By 10 ts expression is detected throughout the gonad (L).

(M–T) Gadd45γ is expressed at high levels until around 25 ts (M–R), and declines thereafter, being undetectable at 13.5 dpc (S and T).

(U–BB) Sry expression profile in the same genetic background.