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. 2012 Nov 13;23(5):1020–1031. doi: 10.1016/j.devcel.2012.09.016

Figure 4.

Figure 4

Genetic Analyses of Functional Interactions between Gadd45γ and Map3k4 during Testis Determination

(A and B) Stra8 expression is ovary specific in wild-type gonads at 14.5 dpc.

(C–O) Significant numbers of Stra8-positive cells are detectable at poles of XY gonads lacking one copy of both Gadd45γ and Map3k4 (E). Only very few Stra8-positive cells are detected, occasionally, in singly heterozygous gonads (C and D). Sox9 and Stra8 WMISH reveal ovary development (H and M), or ovotestis development (I and N), in embryos lacking a single copy of Gadd45γ on C57BL/6J-YAKR (B6-YAKR), in contrast to wild-type controls (G and L). Wild-type XX littermate controls do not express Sox9 (F) but express high levels of Stra8 (K). The presence of a Map3k4 BAC transgene rescues testis development in Gadd45γ+/− heterozygotes (J and O).

(P) Quantitation of gonadal Map3k4 expression by qRT-PCR in wild-type (+/+) and BAC transgenic (+/+ BAC) embryos at 11.5 dpc indicates an approximate 3.5-fold increase of expression in BAC transgenics. Error bars, SEM.