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. 2012 Dec 20;3:472. doi: 10.3389/fphys.2012.00472

Figure 3.

Figure 3

Immunofluorescence (A) and Western blots (B) probed with anti-zyxin antibody showed that zyxin expression was reduced in VSMC stably expressing zyxin-shRNA construct in comparison with cells expressing non-silencing control. Images were taken with a 60x oil objective. Scale bars, 30 μm. Blots were quantified and normalized to β-actin and displayed as percent of control. Control and Zyxin-silenced samples were run on the same immunoblot and were from the same film exposure. The results are representative of three independent experiments.