Figure 8.

Zyxin silencing increased FAK and TRIP6 expression, but decreased Rac1 expression in VSMC. (A) Western blots showed that FAK and TRIP6 expression were increased, but Rac1 was decreased in cells stably expressing zyxin-shRNA compared to cells expressing non-silencing control. Blots were quantified and normalized to β-actin and presented as percentage of the control. (B) Immunofluorescence microscopy showed increased presence of TRIP6 in the focal adhesions of VSMCs expressing zyxin-shRNA, but no significant change was observed in the focal adhesion presence of paxillin and vinculin. Control and Zyxin-silenced samples were run on the same immunoblot and were from the same film exposure. The results are representative of three independent experiments. Scale bars, 30 μm.