Figure 7.

Stratification of the mouse health status, body weight and the genotype at Gdac locus. Forty-two 129 N5 DKO mice were checked for symptoms of wet tail/diarrhea, perianal alopecia, perianal ulceration and lethargy from 8-22 days of age and weighed near 22 days of age. Mice were grouped by the genotype at chromosome 2: 119 Mbp, Gdac locus. In each group, mice were characterized either sick (black portion of the box and whisker plot, N=12) or well (white portion of the plot, N=28). The gray portion of male B6/129 box indicates one well and one sick mouse at 12 gm. Sick mice had consecutive days of symptoms and a median body weight of 9.5 gm (range 7-12 gm, no outliers). Well mice had one or at most two days of intermittent symptoms and a median body weight of 12 gm (range 11-15 gm, one outlier at 9 gm: 1.5X>IQR). Due designation as an outlier by virtue of low body weight, one male B6/B6 mouse was re-classified as sick for association analysis (black circle). The designations by health status and weight corresponded to colon inflammation/pathology scores shown in Fig. 3B.