Fig. 4.
Attenuation of lipid peroxidation–derived 4-hydroxynonenal (4-HNE) (a) and protein nitration-related 3-nitrotyrosine (3-NT) (b) in mitochondria by U-83836E at 12 h following controlled cortical impact traumatic brain injury in male mice. Animals were administered 3 mg/kg of U-83836E i.v. 15 min post-injury and oxidative markers were measured 12 h following injury. The levels of 4-HNE and 3-NT were detected using western blotting, (c) and (d) respectively. Lanes were loaded with either sham (S), vehicle (V), U-83836E (U) or loading control (LC). Values = mean ± SD. Statistical differences (one-way anova and Student-Neuman–Keuls post hoc test): *p < 0.001 versus sham, #p < 0.001 versus vehicle, @ p < 0.05 versus vehicle n = 10.