Skip to main content
. Author manuscript; available in PMC: 2013 Dec 7.
Published in final edited form as: Cell. 2012 Dec 7;151(6):1185–1199. doi: 10.1016/j.cell.2012.10.047

Figure 1. SIRT6 deficient cells are tumorigenic.

Figure 1

A) Sirt6 WT and Sirt6 KO immortalized MEFs (two independent cell lines for each) were plated and cells counted at the indicated times. Errors bars indicate SEM. B) Sirt6 WT and KO cells were plated at very low confluency and assayed for colony formation. C) Two independent immortalized cell lines of the indicated genotypes were injected into the flanks of SCID mice to assess their tumorigenic potential. D) Sirt6 KO immortalized MEFs were transduced with lentiviruses encoding Flag-SIRT6 (WT and HY) and assayed for in vivo tumor formation as in C. E) Anchorage-independent cell growth of Sirt6 WT and KO H-RasV12/shp53 transformed MEFs (error bars indicate SD). F) The same cells as in D were injected into the flanks of SCID mice (n=4) and the tumors harvested and weighted (error bars indicate SD). See also Figure S1.