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. Author manuscript; available in PMC: 2013 Dec 7.
Published in final edited form as: Cell. 2012 Dec 7;151(6):1185–1199. doi: 10.1016/j.cell.2012.10.047

Figure 4. SIRT6 inhibits ribosomal gene expression by co-repressing MYC transcriptional activity.

Figure 4

A) Gene Ontology clustering of SIRT6-bound promoters B) Overlapping of the top 1000 SIRT6- and MYC-bound promoters. C) Gene Set Enrichment Analysis for the overlapping genes described in B. D) H3K4me3, SIRT6 and MYC ChIP signal in the indicated genomic regions in K562 cells and human ES cells (H1). E) Flag-SIRT6 and cMYC IPs showing physical interaction between SIRT6 and MYC. F) Endogenous SIRT6 was immunoprecipitated and the interaction with MYC analyzed by western blot. F) A luciferase reporter gene under the regulation of a MYC-responsive element was contrasfected with empty vector or Flag-SIRT6 plasmids in 293T cells and luciferase expression analyzed 24h later (error bars indicate SEM). G) Expression of the indicated genes in Sirt6 WT and KO H-RasV12/shp53 tumors (n=4) (error bars indicate SEM). I) ChIP analysis of H3K56 acetylation levels in Sirt6 WT and KO H-RasV12/shp53 MEFs (n=4, error bars indicate SEM). See also Figure S4 and Table S1.