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. 2012 Aug 15;21(10):1554–1565. doi: 10.1002/pro.2145

Figure 1.

Figure 1

Titration of 15N-CBD with a spin-labeled minicollagen demonstrates that CBD binds unidirectionally to a minicollagen. A: Overlay of the 1H–15N HSQC spectrum of CBD (black) and the 1H–15N HSQC spectrum of the [(POG)10]3:CBD complex (green) at 1:1 molar ratio shows no differences in chemical shifts of V973, G975, and S979. B: Overlay of the 1H–15N HSQC spectrum of CBD (black) and the 1H–15N HSQC spectrum of the [PROXYL-(POG)6POA(POG)3]3:CBD complex (red) at 1:1 molar ratio shows differences in chemical shifts of V973, G975, and S979. C: Three residues are found distal from Ca2+ ions indicated as green spheres.