Figure5.

Stress response pathways do not mediate osmolyte mediated buffering. (a) Expression levels of GroEL, DnaK and Trigger Factor in presence of osmolytes like TMAO, Proline and glycerol, measured using western-blotting (left panel)quantified and is denoted as bar graph (right panel). (b). color map showing growth of four mutants of glycine-duplet substitution library, transformed in ΔdnaK and ΔotsA strains, at different gentamicin concentrations.(c). Intracellular trehalose concentrations estimated in cells grown at different conditions. Triplicate measurements were made for each growth conditions and the experiments were repeated twice to average the values. (See also Supplementary Figure 28-34).