Abstract
We recently found that bilirubin interferes with the colorimetric chloramphenicol assay of Kakemi et al. (K. T. Kakemi, T. Arita, and S. Ohashi, Yakugaku Zasshi 82:342–345, 1962). Levels of serum bilirubin alone (4 to 6 mg/dl) resulted in apparent concentrations of chloramphenicol which appear to be in the therapeutic range. Concentrations of serum bilirubin greater than 8 mg/dl resulted in levels of apparent chloramphenicol associated with toxicity (>50 μg/ml). Small amounts of activated charcoal added to the isoamyl acetate extraction step of the assay eliminated this interference by bilirubin.
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Selected References
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- Black S. B., Levine P., Shinefield H. R. The necessity for monitoring chloramphenicol levels when treating neonatal meningitis. J Pediatr. 1978 Feb;92(2):235–236. doi: 10.1016/s0022-3476(78)80013-4. [DOI] [PubMed] [Google Scholar]
- Dunkle L. M. Central nervous system chloramphenicol concentration in premature infants. Antimicrob Agents Chemother. 1978 Mar;13(3):427–429. doi: 10.1128/aac.13.3.427. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eichenwald H. F., McCracken G. H., Jr Antimicrobial therapy in infants and children. Part I. Review of antimicrobial agents. J Pediatr. 1978 Sep;93(3):337–356. doi: 10.1016/s0022-3476(78)81139-1. [DOI] [PubMed] [Google Scholar]
- HUGHES D. W., DIAMOND L. K. CHLORAMPHENICOL IN BLOOD: SIMPLE CHEMICAL ESTIMATIONS IN PATIENTS RECEIVING MULTIPLE ANTIBIOTICS. Science. 1964 Apr 17;144(3616):296–297. doi: 10.1126/science.144.3616.296. [DOI] [PubMed] [Google Scholar]
- Jacobsen J., Wennberg R. P. Determination of unbound bilirubin in the serum of newborns. Clin Chem. 1974 Jul;20(7):783–783. [PubMed] [Google Scholar]
- KAKEMI K., ARITA T., OHASHI S. [Absorption and excretion of drugs. V. Determination of chloramphenicol in urine and its metabolic rate]. Yakugaku Zasshi. 1962 Mar;82:345–348. [PubMed] [Google Scholar]
- Koup J. R., Brodsky B., Lau A., Beam T. R., Jr High-performance liquid chromatographic assay of chloramphenicol in serum. Antimicrob Agents Chemother. 1978 Sep;14(3):439–443. doi: 10.1128/aac.14.3.439. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lietman P. S., White T. J., Shaw W. V. Chloramphenicol: an enzymological microassay. Antimicrob Agents Chemother. 1976 Aug;10(2):347–353. doi: 10.1128/aac.10.2.347. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Louie T. J., Tally F. P., Bartlett J. G., Gorbach S. L. Rapid microbiological assay for chloramphenicol and tetracyclines. Antimicrob Agents Chemother. 1976 Jun;9(6):874–878. doi: 10.1128/aac.9.6.874. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Resnick G. L., Corbin D., Sandberg D. H. Determination of serum chloramphenicol utilizing gas--liquid chromatography and electron capture spectrometry. Anal Chem. 1966 Apr;38(4):582–585. doi: 10.1021/ac60236a014. [DOI] [PubMed] [Google Scholar]
- Robison L. R., Seligsohn R., Lerner S. A. Simplified radioenzymatic assay for chloramphenicol. Antimicrob Agents Chemother. 1978 Jan;13(1):25–29. doi: 10.1128/aac.13.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sabath L. D., Casey J. I., Ruch P. A., Stumpf L. L., Finland M. Rapid microassay of gentamicin, kanamycin, neomycin, streptomycin, and vancomycin in serum or plasma. J Lab Clin Med. 1971 Sep;78(3):457–463. [PubMed] [Google Scholar]
- WEISS C. F., GLAZKO A. J., WESTON J. K. Chloramphenicol in the newborn infant. A physiologic explanation of its toxicity when given in excessive doses. N Engl J Med. 1960 Apr 21;262:787–794. doi: 10.1056/NEJM196004212621601. [DOI] [PubMed] [Google Scholar]