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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Free Radic Biol Med. 2012 Jun 19;53(5):1139–1151. doi: 10.1016/j.freeradbiomed.2012.06.012

Figure 6. Targeted decreases in p47phox expression in neuronal cells attenuates neuronal cell death in rat hippocampal slice cultures.

Figure 6

Rat hippocampal slice cultures were transduced with the AAV-SYN-1-p47phoxAS, an AAV-SYN-1null construct, or were untransduced. After 7 days slices were exposed to OGD and the effect on PI uptake (A & B) and LDH release (C) was determined. Representative images are shown. Decreasing p47phox expression reduces PI uptake in the whole slice as well as in the CA1 and DG regions but not in the CA3 (B). Similarly, Decreasing p47phox expression reduces the OGD-mediated increases in LDH release (C). Values are presented as mean ± S.E from 4 independent experiments using 24 pooled slices per experiment. *p<0.05 vs. uninfected no OGD, †p<0.05 vs. OGD exposed untransduced slices.