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Detection of direct and indirect εPKC substrates in total rat heart lysates. Representative 2DE gels (n= 3 hearts of individual animals) of lysates from control hearts (A and D), hearts subjected to, ischemia alone (B and E) and Ischemia + ψεRACK (C and F) as indicated. Coommassie blue G250 stained gels (A-C) and gels stained with phospho-specific dye Pro-Q Diamond (D-F). Spots used to align gels are labeled (A and D).
