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Detection of direct and indirect εPKC substrates in isolated rat heart mitochondria. Representative 2DE gels (n=3 of mitochondria isolated from individual animals) of lysates from control hearts (A and D) and hearts subjected to, Ischemia (B and E) and ψεRACK+ ischemia (C and F) as indicated. Coommassie blue G250 stained gels (A-C) and gels stained with phospho-specific dye Pro-Q Diamond (D-F). Spots used to align gels are labeled (A and D).
