Table 2.
Proteins identified by mass spectrometry whose phosphorylation increased in mitochondria isolated from hearts subjected to ψεRACK+ ischemia relative to ischemia alone. Identified proteins indicated in Figure 6 are shown together with Uniprot accession number, number of peptides identified and, Mascot score, theoretical and experimental molecular weight (M.W.) and 26 isoeletric point. %volume of control (average of three experiments). * P<0.05, as determined by Whitney t-test.
| Spot No. |
Protein | Accession No. | Peptide Count |
Ion Score | Theorical | Experimental | Coverage | Vol (% Ischemia) |
||
|---|---|---|---|---|---|---|---|---|---|---|
| M.W. | pI | M.W. | pI | (%) | ||||||
| 1 | acetyl-CoA dehydrogenase, medium chain | Gi: 8392833 | 9 | 214 | 46kDa | 8.63 | 39kDa | 7.53 | 13 | appeared |
| 2 | sorting and assembly machinery component 50 homolog | gi:51948454 | 4 | 57 | 52kDa | 6.34 | 59KDa | 6.51 | 9 | appeared |
| 3 | dihydrolipoamide dehydrogenase | gi:40786469 | 5 | 102 | 54kDa | 7.96 | 61KDa | 6.43 | 9 | appeared |
| 4 | hydroxysteroid dehydrogenase like 2 [Rattus norvegicus] | gi|71043858 | 3 | 49 | 58KDa | 5.85 | 85KDa | 6.2 | 6 | appeared |
| 5 | protein disulfide-isomerase A3 precursor | gi:1352384 | 8 | 116 | 57kDa | 5.88 | 66KDa | 5.92 | 11 | appeared |
| 6 | protein disulfide-isomerase A3 precursor | gi|1352384 | 10 | 329 | 57KDa | 5.88 | 66KDa | 5.95 | 23 | appeared |
| 7 | aconitase 2 | gi|18079339 | 8 | 163 | 85KDa | 8.05 | 105KDa | 6.4 | 8 | appeared |
| 8 | oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) |
gi|62945278 | 6 | 171 | 12KDa | 6.3 | 174KDa | 5.83 | 8 | appeared |
| 9 | oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) | gi|62945278 | 6 | 44 | 12KDa | 6.3 | 174Kda | 5.93 | 8 | appeared |
| 10 | vimentin | gi|14389299 | 5 | 147 | 54KDa | 5.06 | 67KDa | 4.85 | 5 | appeared |
| 11 | tubulin alpha 1A | gi:38328248 | 4 | 32 | 50kDa | 4.94 | 64KDa | 5.24 | 10 | appeared |
| 12 | tubulin alpha 1A | gi:38328248 | 4 | 36 | 50kDa | 4.94 | 64KDa | 5.31 | 11 | appeared |
| 13 | pyruvate dehydrogenase (lipoamide) beta | gi|56090293 | 5 | 247 | 39KDa | 6.2 | 40KDa | 5.47 | 20 | appeared |
| 14 | branched chain keto acid dehydrogenase E1, beta polypeptide |
gi|158749538 | 4 | 267 | 43KDa | 6.41 | 42KDa | 5.48 | 13 | appeared |
| 15 | striated-muscle alpha tropomyosin | gi|207349 | 9 | 95 | 37KDa | 4.71 | 38KDa | 4.07 | 13 | appeared |
| 19 | mitochondrial aconitase | gi|10637996 | 9 | 196 | 85KDa | 7.87 | 105KDa | 7.16 | 12 | appeared |
| 20 | mitochondrial aconitase | gi|10637996 | 9 | 190 | 85KDa | 7.87 | 105KDa | 7.29 | 12 | appeared |
| 21 | mitochondrial aconitase | gi|10637996 | 8 | 229 | 85KDa | 7.87 | 104KDa | 5.23 | 12 | appeared |
| 22 | mitochondrial aconitase | gi|10637996 | 8 | 325 | 85KDa | 7.87 | 104KDa | 7.71 | 13 | appeared |
| 23 | annexin A2 | gi|9845234 | 8 | 442 | 39KDa | 7.55 | 48KDa | 7.1 | 30 | appeared |
| 24 | aldolase A | gi|202837 | 4 | 125 | 40KDa | 8.3 | 39KDa | 8.04 | 22 | appeared |
| 25 | creatine kinase, mitochondrial 2 | gi|38259206 | 6 | 326 | 47kDa | 8.64 | 46KDa | 7.57 | 21 | appeared |
| 26 | creatine kinase, mitochondrial 2 | gi|38259206 | 9 | 442 | 47kDa | 8.64 | 45KDa | 8.65 | 26 | appeared |
| 27 | acyl-Coenzyme A dehydrogenase, very long chain | gi|6978435 | 7 | 125 | 71KDa | 9.01 | 71KDa | 7.62 | 18 | appeared |
| 28 | acyl-Coenzyme A dehydrogenase, very long chain | gi|6978435 | 5 | 181 | 71KDa | 9.01 | 71KDa | 7.42 | 13 | appeared |
| 29 | 3-oxoacid CoA transferase 1 | gi|189181716 | 8 | 463 | 57kDa | 8.7 | 61KDa | 7.52 | 23 | appeared |
| 30 | 3-oxoacid CoA transferase 1 | gi|189181716 | 4 | 238 | 57kDa | 8.7 | 61KDa | 7.35 | 13 | appeared |
| 31 | ATP synthase alpha subunit precursor | gi|203055 | 8 | 327 | 59KDa | 9.22 | 59KDa | 8.25 | 20 | appeared |
| 32 | pyruvate dehydrogenase E1 alpha form 1 subunit | gi|57657 | 5 | 211 | 43KDa | 8.32 | 66KDa | 6.48 | 7 | appeared |
| 33 | carnitine palmitoyltransferase II | gi|1850592 | 8 | 257 | 74KDa | 7.02 | 74KDa | 7.1 | 7 | appeared |
| 34 | carnitine palmitoyltransferase II | gi|1850592 | 7 | 125 | 74KDa | 7.02 | 74KDa | 7.12 | 15 | appeared |
| 35 | carnitine palmitoyltransferase II | gi|1850592 | 7 | 174 | 74KDa | 7.02 | 74KDa | 7.27 | 13 | appeared |
| 36 | Electron transfer flavoprotein-ubiquinone oxidoreductase | gi|52000614 | 6 | 158 | 61KDa | 7.33 | 70KDa | 7.11 | 15 | appeared |
| 37 | Electron transfer flavoprotein-ubiquinone oxidoreductase | gi|52000614 | 6 | 321 | 61KDa | 7.33 | 70KDa | 7.18 | 14 | appeared |
| 38 | vinculin (predicted), isoform CRA_a | gi|149031250 | 5 | 41 | 123KDa | 5.54 | 146KDa | 5.84 | 5 | appeared |
| 41 | heat shock protein 1, beta (HSP90) | gi|40556608 | 7 | 268 | 83KDa | 4.97 | 105KDa | 4.65 | 9 | appeared |
| 42 | heat shock protein 5 (HSP70 ptn5) glucose regulated protein |
gi|25742763 | 9 | 296 | 72KDa | 5.07 | 84KDa | 4. 7 | 14 | appeared |
| 43 | 70-Kda Heat Shock Cognate Protein | gi|178847300 | 9 | 309 | 60KDa | 5.91 | 72KDa | 5.13 | 20 | appeared |
| 44 | DNAK-type molecular chaperone hsp72-ps1 | gi|347019 | 8 | 369 | 71KDa | 5.43 | 73KDa | 5.21 | 16 | appeared |
| 45 | grp75 | gi|1000439 | 8 | 414 | 74KDa | 5.87 | 79KDa | 5.24 | 16 | appeared |
| 46 | NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa | gi|53850628 | 7 | 283 | 80KDa | 5.65 | 81KDa | 5.14 | 12 | appeared |
| 47 | isocitrate dehydrogenase 3 (NAD+) alpha | gi|16758446 | 7 | 221 | 40KDA | 6.47 | 41KDa | 6.3 | 26 | appeared |
| 48 | succinate dehydrogenase complex, subunit A, flavoprotein (Fp) |
gi|18426858 | 10 | 249 | 72KDa | 6.75 | 72KDa | 6.45 | 20 | appeared |
| 49 | succinate dehydrogenase complex, subunit A, flavoprotein (Fp |
gi|18426858 | 10 | 364 | 72KDa | 6.75 | 71KDa | 6.56 | 19 | appeared |
| 50 | succinate dehydrogenase complex, subunit A, flavoprotein (Fp) |
gi|18426858 | 10 | 355 | 72KDa | 6.75 | 71KDa | 6.82 | 21 | appeared |
| 51 | Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase: Precursor | gi|6015047 | 8 | 219 | 36kDa | 8.13 | 33KDa | 6.47 | 35 | appeared |
| 52 | glyceraldehyde 3-phosphate-dehydrogenase | gi|56188 | 4 | 265 | 36KDa | 8.43 | 47KDa | 7.43 | 3 | appeared |
| 53 | glyceraldehyde 3-phosphate-dehydrogenase | gi|56188 | 3 | 74 | 36kDa | 8.43 | 47KDa | 7.65 | 17 | appeared |
| 54 | ATP synthase beta subunit | gi|1374715 | 6 | 238 | 51KDa | 4.92 | 65KDa | 4.87 | 20 | appeared |
| 55 | tubulin, beta, 2 | gi|5174735 | 6 | 110 | 50kDa | 4.79 | 66KDa | 4.75 | 11 | appeared |
| 56 | Desmin | gi|11968118 | 27 | 65 | 53kDa | 5.21 | 64KDa | 4.87 | 44 | appeared |
| 57 | Desmin | gi|11968118 | 28 | 72 | 53kDa | 5.21 | 64Kda | 5.12 | 53 | appeared |
| 58 | ubiquinol-cytochrome c reductase core protein I | gi|51948476 | 22 | 38 | 53kDa | 5.57 | 51KDa | 5.43 | 32 | appeared |
| 59 | ubiquinol-cytochrome c reductase core protein I | gi|51948476 | 7 | 385 | 53kDa | 5.57 | 52KDa | 5.59 | 21 | appeared |
| 60 | Coq9 protein | gi|51259441 | 2 | 62 | 35KDa | 5.5 | 30KDa | 4.87 | 10 | appeared |
| 61 | Coq9 protein | gi|51259441 | 8 | ND | 35KDa | 5.5 | 30KDa | 5.09 | 25 | 19,5* |
| 62 | polymerase I and transcript release factor | gi:6679567 | 3 | 46 | 44kDa | 5.43 | 64KDa | 3.31 | 7 | 19,5* |