Table 3.

Summary of the function and localization of proteins whose phosphorylation was unique or increased 1.5X (in two out of three gels, of independent samples) in mitochondria from hearts treated with ψεRACK + ischemia relative to ischemia. The biological process, mitochondrial compartment and references to previous descriptions of protein phosphorylation or expression modulated by PKC_ε are indicated in the table.

Function	Protein	Localization	Reference
Fatty Acid oxidation	carnitine palmitoyltransferase II	mitochondrial inner membrane
	delta(3,5)-delta(2,4)-dienoyl-CoA isomerase: precursor	mitochondrial matrix
Glycolysis/ Gluconeogenesis	aldolase A	mitochondrial matrix
Krebs cycle	aconitase 2	mitochondrial matrix
	ATP-specific succinyl-CoA synthase beta subunit	mitochondrial matrix
	isocitrate dehydrogenase 3 (NAD+) alpha	mitochondrial matrix	6, 9
	dihydrolipoamide dehydrogenase (E3)	mitochondrial matrix
	mitochondrial aconitase	mitochondrial matrix
	oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)	mitochondrial matrix	9
	pyruvate dehydrogenase (lipoamide) beta	mitochondrial matrix	9
	pyruvate dehydrogenase E1 alpha form 1 subunit	mitochondrial matrix	9
	glyceraldehyde 3-phosphate-dehydrogenase	mitochondrial matrix	6
Electron transport chain	electron transfer flavoprotein-ubiquinone oxidoreductase	mitochondrial inner membrane
Complex I	NADH dehydrogenase (ubiquinone) Fe-S protein	mitochondrial inner membrane
	electron transfer flavoprotein-ubiquinone oxidoreductase	mitochondrial inner membrane
Complex II	succinate dehydrogenase complex, subunit A, flavoprotein (Fp)	mitochondrial inner membrane	6
	electron transfer flavoprotein-ubiquinone oxidoreductase	mitochondrial inner membrane
Complex III	ubiquinol-cytochrome c reductase core protein I	mitochondrial inner membrane
	electron transfer flavoprotein-ubiquinone oxidoreductase	mitochondrial inner membrane
ATP Synthase	ATP synthase alpha subunit precursor	mitochondrial inner membrane	6
	ATP synthase beta subunit	mitochondrial inner membrane	6, 9
Ketone body metabolism	3-oxoacid CoA transferase 1	mitochondrial matrix
	branched chain keto acid dehydrogenase E1, beta polypeptide	mitochondrial matrix
	vimentin	Cytosol	6, 7
	tubulin alpha 1A	Cytosol
Cytoskeletal elements	tubulin, beta, 2	Cytosol
	desmin	Cytosol	6, 7
	vinculin, isoform CRA_a	Cytosol	6, 7
	heat shock protein 1, beta (HSP90)	Cytosol
Heat Shock Protein	heat shock protein 5 (HSP70 ptn5) glucose regulated protein	Mitochondria
	dnaK-type molecular chaperone hsp72-ps1	Mitochondria	6, 7
	grp75	Mitochondria
Caveoli	polymerase I and transcript release factor (PTRV)	Caveolin
	annexin A2	membranes (Caveolin)	6, 7
	sorting and assembly machinery component 50 homolog	mitochondrion outer membrane
	hydroxysteroid dehydrogenase like 2 [Rattus norvegicus]	mitochondrial inner membrane
Other	protein Coq9 protein	mitochondrial inner membrane
	protein disulfide-isomerase A3 precursor	endoplasmic reticulum
	striated-muscle alpha tropomyosin	Sarcomere