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. Author manuscript; available in PMC: 2012 Dec 20.
Published in final edited form as: Circ J. 2012 Mar 27;76(6):1476–1485. doi: 10.1253/circj.cj-11-1360

Table 4.

Predicted PKC Phosphorylation sites and validated sites of the mitochondrial proteins phosphorylated upon ischemia and ψεRACK. The phosphorylated residue is underlined.

protein predicted p-
site
peptide sequence1 PKC
isoenzyme
Validated2

sorting and assembly machinery component 50 homolog -

T160 LGRAEKVTFQFSYGT PKCδ/ζ
S164 EKVTFQFSYGTKETS cPKC
S171 SYGTKETSYGLSFFK PKCε/δ
S189 GNFEKNFSVNLYKVT PKCζ
S203 TGQFPWSSLRETDRG cPKC
S216 RGVSAEYSFPLCKTS PKCζ
T225 PLCKTSHTVKWEGVW cPKCε/δ
S243 GCLARTASFAVRKES cPKC/ζ
S312 NKPLVLDSVFSTSLW PKCε
S332 PIGDKLSSIADRFYL PKCε

dihydrolipoamide dehydrogenase -

S10 SWSRVYCSLAKKGHF cPKC/ζ
T165 GKNQVTATTADGSTQ PKCε
S170 TATTADGSTQVIGTK PKCδ
S208 VSSTGALSLKKVPEK cPKC
T279 FKLNTKVTGATKKSD cPKC/ζ
T282 NTKVTGATKKSDGKI cPKC
S502 REANLAASFGKPINF cPKC

hydroxysteroid dehydrogenase like 2 -

T12 TGKLAGCTVFITGAS PKCδ
T53 RHPKLLGTIYTAAEE PKCδ/ζ yes
T169 FKQHCAYTIAKYGMS cPKC/ δ/ ζ
S237 SIFKRPKSFTGNFII PKCs/ δ/ ζ
S426 TFRIVKDSLSDEVVR PKCε
S476 DRADVVMSMATEDFV PKCε
T493 FSGKLKPTMAFMSGK cPKC/ζ/ δ/ ε

protein disulfide-isomerase A3 precursor -

S239 IKKFIQESIFGLCPH PKCζ
T228 AYTEKKMTSGKIKKF PKCζ
S229 YTEKKMTSGKIKKFI cPKC
S239 IKKFIQESIFGLCPH PKCδ/ζ
S303 KLNFAVASRKTFSHE cPKC
T306 FAVASRKTFSHELSD PKCδ/ε yes
T452 YEVKGFPTIYFSPAN PKCε
T463 SPANKKLTPKKYEGG cPKC

aconitase 2 -

T64 KRLNRPLTLSEKIVY PKCζ
T366 HPVADVGTVAEKEGW PKCζ
T415 LKCKSQFTITPGSEQ PKCδ/ε
T467 IKKGEKNTIVTSYNR PKCε/ ζ
T504 TALAIAGTLKFNPET cPKC/δ
S690 GRAIITKSFARIHET PKCζ
S770 IEWFRAGSALNRMKE PKCζ

oxoglutarate (alpha-ketoglutarate) dehydrogenase
(lipoamide)
-

T19 RPLTASQTVKTFSQN cPKC/e/d
S71 AWLENPKSVHKSWDI cPKC
S103 PLSLSRSSLATMAHA PKCε/χ/δ yes
T106 LSRSSLATMAHAQSL PKCδ
S112 ATMAHAQSLVEAQPN PKCδ
T190 DKVFHLPTIIFIGGQ PKCδ
T191 KVFHLPIMFIGGQE PKCδ
T262 LARLVRSTRFEEFLQ PKCε
S663 AEYMAFGSLLKEGIH PKCζ
S273 EFLQRKWSSEKRFGL PKCζ
S274 FLQRKWSSEKRFGLE cPKC/δ
S405 TEGKKVMSILLHGDA PKCζ
T437 PSYTTHGTVHVVVNN PKCδ
S861 LIVFTPKSLLRHPEA PKCζ

aldolase A -

S39 AADESTGSIAKRLQS PKCδ/ζ yes
S46 SIAKRLQSIGTENTE PKCε yes
T227 HHVYLEGTLLKPNMV PKCζ
S309 YGRALQASALKAWGG cPKCδ
S336 IKRALANSLACQGKY cPKCδ

acyl-Coenzyme A dehydrogenase, very long chain -

S60 ETLSSDASTREKPAR cPKC/ε
S72 PARAESKSFAVGMFK PKC8/ε
T194 KGILLYGTKAQKEKY PKCζ
S227 SSGSDVASIRSSAVP cPKCδ
S287 TAFVVERSFGGVTHG PKCδ
T347 GRFGMAATLAGTMKA PKCζ
S423 AISKIFGSEAAWKVT PKCζ
S517 RRRTGIGSGLSLSGI PKCζ

3-oxoacid CoA transferase 1 -

S16 SGLRLCASARNSRGA cPKC
S35 CACYFSVSTRHHTKF cPKC
T58 KDIPNGATLLVGGFG PKCδ
T140 VELTPQGTLAERIRA PKCζ
T163 YTSTGYGTLVQEGGS PKCε
S179 IKYNKDGSVAIASKP PKCε/ζ/δ
S253 EEIVDIGSFAPEDIH PKCε
S283 EKRIERLSLRKEGEG cPKC/ε/δ/ζ
T397 RGGHVNLTMLGAMQV PKCζ
T440 SKTKVVVTMEHSAKG cPKC/ε
T457 HKIMEKCTLPLTGKQ cPKCδ

ATP synthase alpha subunit precursor -

T102 ITPETFSTISVVGLI PKCδ

pyruvate dehydrogenase E1 alpha form 1 subunit -

T35 RNFANDATFEIKKCD PKCζ
T70 KYYRMMQTVRRMELK cPKC/ε
T124 AYRAHGFTFNRGHAV PKCδ
T139 RAILAELTGRRGGCA PKCδ
S152 CAKGKGGSMHMYAKN PKCδ/ζ
T266 ILCVREATKFAAAYC PKCδ
S293 TYRYHGHSMSDPGVS PKCε yes

carnitine palmitoyltransferase II -

S15 RAWPRCPSLVLGAPS PKCδ
T60 PIPKLEDTMKRYLNA cPKC
T156 LTRATNLTVSAVRFL PKCδ
S320 ETLKKVDSAVFCLCL PKCζ
S411 AATNSSASVETLSFN PKCδ
S416 SASVETLSFNLSGAL PKCδ
T428 GALKAGITAAKEKFD PKCζ
T437 AKEKFDTTVKTLSID PKCε/δ/χ
S462 FLKKKQLSPDAVAQL PKCδ
T491 ATYESCSTAAFKHGR PKCζ
T501 FKHGRTETIRPASIF cPKC
S513 SIFTKRCSEAFVRDP PKCζ

Electron transfer flavoprotein-ubiquinone oxidoreductase -

T46 PQITTHYTIHPREKD cPKC
T229 KDGAPKTTFERGLEL PKCδ
T241 LELHAKVTIFAEGCH PKCε/δ
S306 DRHTYGGSFLYHLNE PKCζ
S347 QRWKHHPSIRPTLEG cPKC/δ
T401 PKIKGTHTAMKSGSL PKCε/δ/ζ
S407 HTAMKSGSLAAEAIF PKCε/δ
S490 WTLKHKGSDSEQLKP cPKC/ε
S550 IPVNRNLSIYDGPEQ PKCζ yes

NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa -

S69 RPLTTSMSLFIIAPT PKCε/ζ
S110 PFILATSSLSVYSIL PKCε
S128 WASNSKYSLFGALRA PKCε/δ
T139 ALRAVAQTISYEVTM PKCδ
S258 YPELYSTSFMTETLL PKCε
S276 TFLWIRASYPRFRYD cPKC
T297 WKNFLPLTLAFCMWY PKCζ

isocitrate dehydrogenase 3 (NAD+) alpha -

S340 ATIKDGKSLTKDLGG PKCδ/ζ
T334 IEAACFATIKDGKSL cPKC/δ

succinate dehydrogenase complex, subunit A,
flavoprotein (Fp)
-

S28 ATRGFHFSVGESKKA cPKC
S36 VGESKKASAKVSDAI PKCδ
T118 WRWHFYDTVKGSDWL cPKC
S169 QRAFGGQSLKFGKGG cPKC/δ/ζ
S206 RSLRYDTSYFVEYFA PKCε/ζ
T244 HRIRAKNTIIATGGY cPKC/ε/δ/ζ
S462 FGRACALSIAESCRP cPKC/δ
S466 CALSIAESCRPGDKV cPKC
S484 KANAGEESVMNLDKL PKCδ
S497 KLRFADGSVRTSELR PKCε/χ/δ/ζ
S506 RTSELRLSMQKSMQS cPKC
S510 LRLSMQKSMQSHAAV PKCδ/ζ
S522 AAVFRVGSVLQEGCE PKCδ/ζ yes
T618 AEHWRKHTLSYVDTK PKCε/δ/ζ
S620 HWRKHTLSYVDTKTG cPKC/ζ
T630 DTKTGKVTLDYRPVI PKCε
T640 YRPVIDKTLNEADCA PKCε

Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase: Precursor -

S30 RQLYFNVSLRSLSSS cPKC/ζ
T153 SRYQKTFTVIEKCPK PKCε/ζ
T225 RSLVNELTFTARKMM PKCδ

glyceraldehyde 3-phosphate-dehydrogenase -

T57 THGKFNGTVKAENGK cPKC/ε yes
T185 AITATQKTVDGPSGK PKCδ yes
T292 NSNSHSSTFDAGAGI PKCε/δ

ubiquinol-cytochrome c reductase core protein I -

S107 TKSSKESSEARKGFS PKCε/δ
T120 FSYLVTAI IIVGVAY PKCδ
T122 YLVTAIIIVGVAYAA PKCε
T180 PLFVRHRTKKEIDQE cPKC

pyruvate dehydrogenase (lipoamide) alpha -

T35 RNFANDATFEIKKCD PKCζ
T70 KYYRMMQTVRRMELK cPKC/ε
T124 AYRAHGFTFNRGHAV PKCδ
T139 RAILAELTGRRGGCA PKCδ
S152 CAKGKGGSMHMYAKN PKCδ/ζ
T266 ILCVREATKFAAAYC PKCδ
S293 TYRYHGHSMSDPGVS PKCε

pyruvate dehydrogenase (lipoamide) beta -

S16 RGPLRQASGLLKRRF PKCζ
T112 RPICEFMTFNFSMQA PKCζ
T235 AKIERQGTHITVVAH PKCζ
S282 DIEAIEASVMKTNHL PKCδ

ATP synthase beta subunit -

S51 RDYAAQSSAAPKAGT PKCζ
S231 AKAHGGYSVFAGVGE PKCζ
T288 RVALTGLTVAEYFRD PKCζ
S353 IIIIKKGSITSVQAI PKCδ/ε/χ

Branched chain keto acid dehydrogenase E1, beta
polypeptide
-

T105 FGGVFRCTVGLRDKY cPKC
S177 GDLFNCGSLTIRAPW cPKC
1

Predicted by Scansite (http://scansite.mit.edu).

2

Valic ated sites reported in phosphosite (http//www.phosphosite.org).