Table 4.
Predicted PKC Phosphorylation sites and validated sites of the mitochondrial proteins phosphorylated upon ischemia and ψεRACK. The phosphorylated residue is underlined.
| protein | predicted p- site |
peptide sequence1 | PKC isoenzyme |
Validated2 |
|---|---|---|---|---|
|
| ||||
| sorting and assembly machinery component 50 homolog | - | |||
|
| ||||
| T160 | LGRAEKVTFQFSYGT | PKCδ/ζ | ||
| S164 | EKVTFQFSYGTKETS | cPKC | ||
| S171 | SYGTKETSYGLSFFK | PKCε/δ | ||
| S189 | GNFEKNFSVNLYKVT | PKCζ | ||
| S203 | TGQFPWSSLRETDRG | cPKC | ||
| S216 | RGVSAEYSFPLCKTS | PKCζ | ||
| T225 | PLCKTSHTVKWEGVW | cPKCε/δ | ||
| S243 | GCLARTASFAVRKES | cPKC/ζ | ||
| S312 | NKPLVLDSVFSTSLW | PKCε | ||
| S332 | PIGDKLSSIADRFYL | PKCε | ||
|
| ||||
| dihydrolipoamide dehydrogenase | - | |||
|
| ||||
| S10 | SWSRVYCSLAKKGHF | cPKC/ζ | ||
| T165 | GKNQVTATTADGSTQ | PKCε | ||
| S170 | TATTADGSTQVIGTK | PKCδ | ||
| S208 | VSSTGALSLKKVPEK | cPKC | ||
| T279 | FKLNTKVTGATKKSD | cPKC/ζ | ||
| T282 | NTKVTGATKKSDGKI | cPKC | ||
| S502 | REANLAASFGKPINF | cPKC | ||
|
| ||||
| hydroxysteroid dehydrogenase like 2 | - | |||
|
| ||||
| T12 | TGKLAGCTVFITGAS | PKCδ | ||
| T53 | RHPKLLGTIYTAAEE | PKCδ/ζ | yes | |
| T169 | FKQHCAYTIAKYGMS | cPKC/ δ/ ζ | ||
| S237 | SIFKRPKSFTGNFII | PKCs/ δ/ ζ | ||
| S426 | TFRIVKDSLSDEVVR | PKCε | ||
| S476 | DRADVVMSMATEDFV | PKCε | ||
| T493 | FSGKLKPTMAFMSGK | cPKC/ζ/ δ/ ε | ||
|
| ||||
| protein disulfide-isomerase A3 precursor | - | |||
|
| ||||
| S239 | IKKFIQESIFGLCPH | PKCζ | ||
| T228 | AYTEKKMTSGKIKKF | PKCζ | ||
| S229 | YTEKKMTSGKIKKFI | cPKC | ||
| S239 | IKKFIQESIFGLCPH | PKCδ/ζ | ||
| S303 | KLNFAVASRKTFSHE | cPKC | ||
| T306 | FAVASRKTFSHELSD | PKCδ/ε | yes | |
| T452 | YEVKGFPTIYFSPAN | PKCε | ||
| T463 | SPANKKLTPKKYEGG | cPKC | ||
|
| ||||
| aconitase 2 | - | |||
|
| ||||
| T64 | KRLNRPLTLSEKIVY | PKCζ | ||
| T366 | HPVADVGTVAEKEGW | PKCζ | ||
| T415 | LKCKSQFTITPGSEQ | PKCδ/ε | ||
| T467 | IKKGEKNTIVTSYNR | PKCε/ ζ | ||
| T504 | TALAIAGTLKFNPET | cPKC/δ | ||
| S690 | GRAIITKSFARIHET | PKCζ | ||
| S770 | IEWFRAGSALNRMKE | PKCζ | ||
|
| ||||
|
oxoglutarate (alpha-ketoglutarate) dehydrogenase
(lipoamide) |
- | |||
|
| ||||
| T19 | RPLTASQTVKTFSQN | cPKC/e/d | ||
| S71 | AWLENPKSVHKSWDI | cPKC | ||
| S103 | PLSLSRSSLATMAHA | PKCε/χ/δ | yes | |
| T106 | LSRSSLATMAHAQSL | PKCδ | ||
| S112 | ATMAHAQSLVEAQPN | PKCδ | ||
| T190 | DKVFHLPTIIFIGGQ | PKCδ | ||
| T191 | KVFHLPIMFIGGQE | PKCδ | ||
| T262 | LARLVRSTRFEEFLQ | PKCε | ||
| S663 | AEYMAFGSLLKEGIH | PKCζ | ||
| S273 | EFLQRKWSSEKRFGL | PKCζ | ||
| S274 | FLQRKWSSEKRFGLE | cPKC/δ | ||
| S405 | TEGKKVMSILLHGDA | PKCζ | ||
| T437 | PSYTTHGTVHVVVNN | PKCδ | ||
| S861 | LIVFTPKSLLRHPEA | PKCζ | ||
|
| ||||
| aldolase A | - | |||
|
| ||||
| S39 | AADESTGSIAKRLQS | PKCδ/ζ | yes | |
| S46 | SIAKRLQSIGTENTE | PKCε | yes | |
| T227 | HHVYLEGTLLKPNMV | PKCζ | ||
| S309 | YGRALQASALKAWGG | cPKCδ | ||
| S336 | IKRALANSLACQGKY | cPKCδ | ||
|
| ||||
| acyl-Coenzyme A dehydrogenase, very long chain | - | |||
|
| ||||
| S60 | ETLSSDASTREKPAR | cPKC/ε | ||
| S72 | PARAESKSFAVGMFK | PKC8/ε | ||
| T194 | KGILLYGTKAQKEKY | PKCζ | ||
| S227 | SSGSDVASIRSSAVP | cPKCδ | ||
| S287 | TAFVVERSFGGVTHG | PKCδ | ||
| T347 | GRFGMAATLAGTMKA | PKCζ | ||
| S423 | AISKIFGSEAAWKVT | PKCζ | ||
| S517 | RRRTGIGSGLSLSGI | PKCζ | ||
|
| ||||
| 3-oxoacid CoA transferase 1 | - | |||
|
| ||||
| S16 | SGLRLCASARNSRGA | cPKC | ||
| S35 | CACYFSVSTRHHTKF | cPKC | ||
| T58 | KDIPNGATLLVGGFG | PKCδ | ||
| T140 | VELTPQGTLAERIRA | PKCζ | ||
| T163 | YTSTGYGTLVQEGGS | PKCε | ||
| S179 | IKYNKDGSVAIASKP | PKCε/ζ/δ | ||
| S253 | EEIVDIGSFAPEDIH | PKCε | ||
| S283 | EKRIERLSLRKEGEG | cPKC/ε/δ/ζ | ||
| T397 | RGGHVNLTMLGAMQV | PKCζ | ||
| T440 | SKTKVVVTMEHSAKG | cPKC/ε | ||
| T457 | HKIMEKCTLPLTGKQ | cPKCδ | ||
|
| ||||
| ATP synthase alpha subunit precursor | - | |||
|
| ||||
| T102 | ITPETFSTISVVGLI | PKCδ | ||
|
| ||||
| pyruvate dehydrogenase E1 alpha form 1 subunit | - | |||
|
| ||||
| T35 | RNFANDATFEIKKCD | PKCζ | ||
| T70 | KYYRMMQTVRRMELK | cPKC/ε | ||
| T124 | AYRAHGFTFNRGHAV | PKCδ | ||
| T139 | RAILAELTGRRGGCA | PKCδ | ||
| S152 | CAKGKGGSMHMYAKN | PKCδ/ζ | ||
| T266 | ILCVREATKFAAAYC | PKCδ | ||
| S293 | TYRYHGHSMSDPGVS | PKCε | yes | |
|
| ||||
| carnitine palmitoyltransferase II | - | |||
|
| ||||
| S15 | RAWPRCPSLVLGAPS | PKCδ | ||
| T60 | PIPKLEDTMKRYLNA | cPKC | ||
| T156 | LTRATNLTVSAVRFL | PKCδ | ||
| S320 | ETLKKVDSAVFCLCL | PKCζ | ||
| S411 | AATNSSASVETLSFN | PKCδ | ||
| S416 | SASVETLSFNLSGAL | PKCδ | ||
| T428 | GALKAGITAAKEKFD | PKCζ | ||
| T437 | AKEKFDTTVKTLSID | PKCε/δ/χ | ||
| S462 | FLKKKQLSPDAVAQL | PKCδ | ||
| T491 | ATYESCSTAAFKHGR | PKCζ | ||
| T501 | FKHGRTETIRPASIF | cPKC | ||
| S513 | SIFTKRCSEAFVRDP | PKCζ | ||
|
| ||||
| Electron transfer flavoprotein-ubiquinone oxidoreductase | - | |||
|
| ||||
| T46 | PQITTHYTIHPREKD | cPKC | ||
| T229 | KDGAPKTTFERGLEL | PKCδ | ||
| T241 | LELHAKVTIFAEGCH | PKCε/δ | ||
| S306 | DRHTYGGSFLYHLNE | PKCζ | ||
| S347 | QRWKHHPSIRPTLEG | cPKC/δ | ||
| T401 | PKIKGTHTAMKSGSL | PKCε/δ/ζ | ||
| S407 | HTAMKSGSLAAEAIF | PKCε/δ | ||
| S490 | WTLKHKGSDSEQLKP | cPKC/ε | ||
| S550 | IPVNRNLSIYDGPEQ | PKCζ | yes | |
|
| ||||
| NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa | - | |||
|
| ||||
| S69 | RPLTTSMSLFIIAPT | PKCε/ζ | ||
| S110 | PFILATSSLSVYSIL | PKCε | ||
| S128 | WASNSKYSLFGALRA | PKCε/δ | ||
| T139 | ALRAVAQTISYEVTM | PKCδ | ||
| S258 | YPELYSTSFMTETLL | PKCε | ||
| S276 | TFLWIRASYPRFRYD | cPKC | ||
| T297 | WKNFLPLTLAFCMWY | PKCζ | ||
|
| ||||
| isocitrate dehydrogenase 3 (NAD+) alpha | - | |||
|
| ||||
| S340 | ATIKDGKSLTKDLGG | PKCδ/ζ | ||
| T334 | IEAACFATIKDGKSL | cPKC/δ | ||
|
| ||||
|
succinate dehydrogenase complex, subunit A,
flavoprotein (Fp) |
- | |||
|
| ||||
| S28 | ATRGFHFSVGESKKA | cPKC | ||
| S36 | VGESKKASAKVSDAI | PKCδ | ||
| T118 | WRWHFYDTVKGSDWL | cPKC | ||
| S169 | QRAFGGQSLKFGKGG | cPKC/δ/ζ | ||
| S206 | RSLRYDTSYFVEYFA | PKCε/ζ | ||
| T244 | HRIRAKNTIIATGGY | cPKC/ε/δ/ζ | ||
| S462 | FGRACALSIAESCRP | cPKC/δ | ||
| S466 | CALSIAESCRPGDKV | cPKC | ||
| S484 | KANAGEESVMNLDKL | PKCδ | ||
| S497 | KLRFADGSVRTSELR | PKCε/χ/δ/ζ | ||
| S506 | RTSELRLSMQKSMQS | cPKC | ||
| S510 | LRLSMQKSMQSHAAV | PKCδ/ζ | ||
| S522 | AAVFRVGSVLQEGCE | PKCδ/ζ | yes | |
| T618 | AEHWRKHTLSYVDTK | PKCε/δ/ζ | ||
| S620 | HWRKHTLSYVDTKTG | cPKC/ζ | ||
| T630 | DTKTGKVTLDYRPVI | PKCε | ||
| T640 | YRPVIDKTLNEADCA | PKCε | ||
|
| ||||
| Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase: Precursor | - | |||
|
| ||||
| S30 | RQLYFNVSLRSLSSS | cPKC/ζ | ||
| T153 | SRYQKTFTVIEKCPK | PKCε/ζ | ||
| T225 | RSLVNELTFTARKMM | PKCδ | ||
|
| ||||
| glyceraldehyde 3-phosphate-dehydrogenase | - | |||
|
| ||||
| T57 | THGKFNGTVKAENGK | cPKC/ε | yes | |
| T185 | AITATQKTVDGPSGK | PKCδ | yes | |
| T292 | NSNSHSSTFDAGAGI | PKCε/δ | ||
|
| ||||
| ubiquinol-cytochrome c reductase core protein I | - | |||
|
| ||||
| S107 | TKSSKESSEARKGFS | PKCε/δ | ||
| T120 | FSYLVTAI IIVGVAY | PKCδ | ||
| T122 | YLVTAIIIVGVAYAA | PKCε | ||
| T180 | PLFVRHRTKKEIDQE | cPKC | ||
|
| ||||
| pyruvate dehydrogenase (lipoamide) alpha | - | |||
|
| ||||
| T35 | RNFANDATFEIKKCD | PKCζ | ||
| T70 | KYYRMMQTVRRMELK | cPKC/ε | ||
| T124 | AYRAHGFTFNRGHAV | PKCδ | ||
| T139 | RAILAELTGRRGGCA | PKCδ | ||
| S152 | CAKGKGGSMHMYAKN | PKCδ/ζ | ||
| T266 | ILCVREATKFAAAYC | PKCδ | ||
| S293 | TYRYHGHSMSDPGVS | PKCε | ||
|
| ||||
| pyruvate dehydrogenase (lipoamide) beta | - | |||
|
| ||||
| S16 | RGPLRQASGLLKRRF | PKCζ | ||
| T112 | RPICEFMTFNFSMQA | PKCζ | ||
| T235 | AKIERQGTHITVVAH | PKCζ | ||
| S282 | DIEAIEASVMKTNHL | PKCδ | ||
|
| ||||
| ATP synthase beta subunit | - | |||
|
| ||||
| S51 | RDYAAQSSAAPKAGT | PKCζ | ||
| S231 | AKAHGGYSVFAGVGE | PKCζ | ||
| T288 | RVALTGLTVAEYFRD | PKCζ | ||
| S353 | IIIIKKGSITSVQAI | PKCδ/ε/χ | ||
|
| ||||
|
Branched chain keto acid dehydrogenase E1, beta
polypeptide |
- | |||
|
| ||||
| T105 | FGGVFRCTVGLRDKY | cPKC | ||
| S177 | GDLFNCGSLTIRAPW | cPKC | ||
1
Predicted by Scansite (http://scansite.mit.edu).
2
Valic ated sites reported in phosphosite (http//www.phosphosite.org).