Table 4.
protein | predicted p- site |
peptide sequence1 | PKC isoenzyme |
Validated2 |
---|---|---|---|---|
| ||||
sorting and assembly machinery component 50 homolog | - | |||
| ||||
T160 | LGRAEKVTFQFSYGT | PKCδ/ζ | ||
S164 | EKVTFQFSYGTKETS | cPKC | ||
S171 | SYGTKETSYGLSFFK | PKCε/δ | ||
S189 | GNFEKNFSVNLYKVT | PKCζ | ||
S203 | TGQFPWSSLRETDRG | cPKC | ||
S216 | RGVSAEYSFPLCKTS | PKCζ | ||
T225 | PLCKTSHTVKWEGVW | cPKCε/δ | ||
S243 | GCLARTASFAVRKES | cPKC/ζ | ||
S312 | NKPLVLDSVFSTSLW | PKCε | ||
S332 | PIGDKLSSIADRFYL | PKCε | ||
| ||||
dihydrolipoamide dehydrogenase | - | |||
| ||||
S10 | SWSRVYCSLAKKGHF | cPKC/ζ | ||
T165 | GKNQVTATTADGSTQ | PKCε | ||
S170 | TATTADGSTQVIGTK | PKCδ | ||
S208 | VSSTGALSLKKVPEK | cPKC | ||
T279 | FKLNTKVTGATKKSD | cPKC/ζ | ||
T282 | NTKVTGATKKSDGKI | cPKC | ||
S502 | REANLAASFGKPINF | cPKC | ||
| ||||
hydroxysteroid dehydrogenase like 2 | - | |||
| ||||
T12 | TGKLAGCTVFITGAS | PKCδ | ||
T53 | RHPKLLGTIYTAAEE | PKCδ/ζ | yes | |
T169 | FKQHCAYTIAKYGMS | cPKC/ δ/ ζ | ||
S237 | SIFKRPKSFTGNFII | PKCs/ δ/ ζ | ||
S426 | TFRIVKDSLSDEVVR | PKCε | ||
S476 | DRADVVMSMATEDFV | PKCε | ||
T493 | FSGKLKPTMAFMSGK | cPKC/ζ/ δ/ ε | ||
| ||||
protein disulfide-isomerase A3 precursor | - | |||
| ||||
S239 | IKKFIQESIFGLCPH | PKCζ | ||
T228 | AYTEKKMTSGKIKKF | PKCζ | ||
S229 | YTEKKMTSGKIKKFI | cPKC | ||
S239 | IKKFIQESIFGLCPH | PKCδ/ζ | ||
S303 | KLNFAVASRKTFSHE | cPKC | ||
T306 | FAVASRKTFSHELSD | PKCδ/ε | yes | |
T452 | YEVKGFPTIYFSPAN | PKCε | ||
T463 | SPANKKLTPKKYEGG | cPKC | ||
| ||||
aconitase 2 | - | |||
| ||||
T64 | KRLNRPLTLSEKIVY | PKCζ | ||
T366 | HPVADVGTVAEKEGW | PKCζ | ||
T415 | LKCKSQFTITPGSEQ | PKCδ/ε | ||
T467 | IKKGEKNTIVTSYNR | PKCε/ ζ | ||
T504 | TALAIAGTLKFNPET | cPKC/δ | ||
S690 | GRAIITKSFARIHET | PKCζ | ||
S770 | IEWFRAGSALNRMKE | PKCζ | ||
| ||||
oxoglutarate (alpha-ketoglutarate) dehydrogenase
(lipoamide) |
- | |||
| ||||
T19 | RPLTASQTVKTFSQN | cPKC/e/d | ||
S71 | AWLENPKSVHKSWDI | cPKC | ||
S103 | PLSLSRSSLATMAHA | PKCε/χ/δ | yes | |
T106 | LSRSSLATMAHAQSL | PKCδ | ||
S112 | ATMAHAQSLVEAQPN | PKCδ | ||
T190 | DKVFHLPTIIFIGGQ | PKCδ | ||
T191 | KVFHLPIMFIGGQE | PKCδ | ||
T262 | LARLVRSTRFEEFLQ | PKCε | ||
S663 | AEYMAFGSLLKEGIH | PKCζ | ||
S273 | EFLQRKWSSEKRFGL | PKCζ | ||
S274 | FLQRKWSSEKRFGLE | cPKC/δ | ||
S405 | TEGKKVMSILLHGDA | PKCζ | ||
T437 | PSYTTHGTVHVVVNN | PKCδ | ||
S861 | LIVFTPKSLLRHPEA | PKCζ | ||
| ||||
aldolase A | - | |||
| ||||
S39 | AADESTGSIAKRLQS | PKCδ/ζ | yes | |
S46 | SIAKRLQSIGTENTE | PKCε | yes | |
T227 | HHVYLEGTLLKPNMV | PKCζ | ||
S309 | YGRALQASALKAWGG | cPKCδ | ||
S336 | IKRALANSLACQGKY | cPKCδ | ||
| ||||
acyl-Coenzyme A dehydrogenase, very long chain | - | |||
| ||||
S60 | ETLSSDASTREKPAR | cPKC/ε | ||
S72 | PARAESKSFAVGMFK | PKC8/ε | ||
T194 | KGILLYGTKAQKEKY | PKCζ | ||
S227 | SSGSDVASIRSSAVP | cPKCδ | ||
S287 | TAFVVERSFGGVTHG | PKCδ | ||
T347 | GRFGMAATLAGTMKA | PKCζ | ||
S423 | AISKIFGSEAAWKVT | PKCζ | ||
S517 | RRRTGIGSGLSLSGI | PKCζ | ||
| ||||
3-oxoacid CoA transferase 1 | - | |||
| ||||
S16 | SGLRLCASARNSRGA | cPKC | ||
S35 | CACYFSVSTRHHTKF | cPKC | ||
T58 | KDIPNGATLLVGGFG | PKCδ | ||
T140 | VELTPQGTLAERIRA | PKCζ | ||
T163 | YTSTGYGTLVQEGGS | PKCε | ||
S179 | IKYNKDGSVAIASKP | PKCε/ζ/δ | ||
S253 | EEIVDIGSFAPEDIH | PKCε | ||
S283 | EKRIERLSLRKEGEG | cPKC/ε/δ/ζ | ||
T397 | RGGHVNLTMLGAMQV | PKCζ | ||
T440 | SKTKVVVTMEHSAKG | cPKC/ε | ||
T457 | HKIMEKCTLPLTGKQ | cPKCδ | ||
| ||||
ATP synthase alpha subunit precursor | - | |||
| ||||
T102 | ITPETFSTISVVGLI | PKCδ | ||
| ||||
pyruvate dehydrogenase E1 alpha form 1 subunit | - | |||
| ||||
T35 | RNFANDATFEIKKCD | PKCζ | ||
T70 | KYYRMMQTVRRMELK | cPKC/ε | ||
T124 | AYRAHGFTFNRGHAV | PKCδ | ||
T139 | RAILAELTGRRGGCA | PKCδ | ||
S152 | CAKGKGGSMHMYAKN | PKCδ/ζ | ||
T266 | ILCVREATKFAAAYC | PKCδ | ||
S293 | TYRYHGHSMSDPGVS | PKCε | yes | |
| ||||
carnitine palmitoyltransferase II | - | |||
| ||||
S15 | RAWPRCPSLVLGAPS | PKCδ | ||
T60 | PIPKLEDTMKRYLNA | cPKC | ||
T156 | LTRATNLTVSAVRFL | PKCδ | ||
S320 | ETLKKVDSAVFCLCL | PKCζ | ||
S411 | AATNSSASVETLSFN | PKCδ | ||
S416 | SASVETLSFNLSGAL | PKCδ | ||
T428 | GALKAGITAAKEKFD | PKCζ | ||
T437 | AKEKFDTTVKTLSID | PKCε/δ/χ | ||
S462 | FLKKKQLSPDAVAQL | PKCδ | ||
T491 | ATYESCSTAAFKHGR | PKCζ | ||
T501 | FKHGRTETIRPASIF | cPKC | ||
S513 | SIFTKRCSEAFVRDP | PKCζ | ||
| ||||
Electron transfer flavoprotein-ubiquinone oxidoreductase | - | |||
| ||||
T46 | PQITTHYTIHPREKD | cPKC | ||
T229 | KDGAPKTTFERGLEL | PKCδ | ||
T241 | LELHAKVTIFAEGCH | PKCε/δ | ||
S306 | DRHTYGGSFLYHLNE | PKCζ | ||
S347 | QRWKHHPSIRPTLEG | cPKC/δ | ||
T401 | PKIKGTHTAMKSGSL | PKCε/δ/ζ | ||
S407 | HTAMKSGSLAAEAIF | PKCε/δ | ||
S490 | WTLKHKGSDSEQLKP | cPKC/ε | ||
S550 | IPVNRNLSIYDGPEQ | PKCζ | yes | |
| ||||
NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa | - | |||
| ||||
S69 | RPLTTSMSLFIIAPT | PKCε/ζ | ||
S110 | PFILATSSLSVYSIL | PKCε | ||
S128 | WASNSKYSLFGALRA | PKCε/δ | ||
T139 | ALRAVAQTISYEVTM | PKCδ | ||
S258 | YPELYSTSFMTETLL | PKCε | ||
S276 | TFLWIRASYPRFRYD | cPKC | ||
T297 | WKNFLPLTLAFCMWY | PKCζ | ||
| ||||
isocitrate dehydrogenase 3 (NAD+) alpha | - | |||
| ||||
S340 | ATIKDGKSLTKDLGG | PKCδ/ζ | ||
T334 | IEAACFATIKDGKSL | cPKC/δ | ||
| ||||
succinate dehydrogenase complex, subunit A,
flavoprotein (Fp) |
- | |||
| ||||
S28 | ATRGFHFSVGESKKA | cPKC | ||
S36 | VGESKKASAKVSDAI | PKCδ | ||
T118 | WRWHFYDTVKGSDWL | cPKC | ||
S169 | QRAFGGQSLKFGKGG | cPKC/δ/ζ | ||
S206 | RSLRYDTSYFVEYFA | PKCε/ζ | ||
T244 | HRIRAKNTIIATGGY | cPKC/ε/δ/ζ | ||
S462 | FGRACALSIAESCRP | cPKC/δ | ||
S466 | CALSIAESCRPGDKV | cPKC | ||
S484 | KANAGEESVMNLDKL | PKCδ | ||
S497 | KLRFADGSVRTSELR | PKCε/χ/δ/ζ | ||
S506 | RTSELRLSMQKSMQS | cPKC | ||
S510 | LRLSMQKSMQSHAAV | PKCδ/ζ | ||
S522 | AAVFRVGSVLQEGCE | PKCδ/ζ | yes | |
T618 | AEHWRKHTLSYVDTK | PKCε/δ/ζ | ||
S620 | HWRKHTLSYVDTKTG | cPKC/ζ | ||
T630 | DTKTGKVTLDYRPVI | PKCε | ||
T640 | YRPVIDKTLNEADCA | PKCε | ||
| ||||
Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase: Precursor | - | |||
| ||||
S30 | RQLYFNVSLRSLSSS | cPKC/ζ | ||
T153 | SRYQKTFTVIEKCPK | PKCε/ζ | ||
T225 | RSLVNELTFTARKMM | PKCδ | ||
| ||||
glyceraldehyde 3-phosphate-dehydrogenase | - | |||
| ||||
T57 | THGKFNGTVKAENGK | cPKC/ε | yes | |
T185 | AITATQKTVDGPSGK | PKCδ | yes | |
T292 | NSNSHSSTFDAGAGI | PKCε/δ | ||
| ||||
ubiquinol-cytochrome c reductase core protein I | - | |||
| ||||
S107 | TKSSKESSEARKGFS | PKCε/δ | ||
T120 | FSYLVTAI IIVGVAY | PKCδ | ||
T122 | YLVTAIIIVGVAYAA | PKCε | ||
T180 | PLFVRHRTKKEIDQE | cPKC | ||
| ||||
pyruvate dehydrogenase (lipoamide) alpha | - | |||
| ||||
T35 | RNFANDATFEIKKCD | PKCζ | ||
T70 | KYYRMMQTVRRMELK | cPKC/ε | ||
T124 | AYRAHGFTFNRGHAV | PKCδ | ||
T139 | RAILAELTGRRGGCA | PKCδ | ||
S152 | CAKGKGGSMHMYAKN | PKCδ/ζ | ||
T266 | ILCVREATKFAAAYC | PKCδ | ||
S293 | TYRYHGHSMSDPGVS | PKCε | ||
| ||||
pyruvate dehydrogenase (lipoamide) beta | - | |||
| ||||
S16 | RGPLRQASGLLKRRF | PKCζ | ||
T112 | RPICEFMTFNFSMQA | PKCζ | ||
T235 | AKIERQGTHITVVAH | PKCζ | ||
S282 | DIEAIEASVMKTNHL | PKCδ | ||
| ||||
ATP synthase beta subunit | - | |||
| ||||
S51 | RDYAAQSSAAPKAGT | PKCζ | ||
S231 | AKAHGGYSVFAGVGE | PKCζ | ||
T288 | RVALTGLTVAEYFRD | PKCζ | ||
S353 | IIIIKKGSITSVQAI | PKCδ/ε/χ | ||
| ||||
Branched chain keto acid dehydrogenase E1, beta
polypeptide |
- | |||
| ||||
T105 | FGGVFRCTVGLRDKY | cPKC | ||
S177 | GDLFNCGSLTIRAPW | cPKC |
Predicted by Scansite (http://scansite.mit.edu).
Valic ated sites reported in phosphosite (http//www.phosphosite.org).