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. Author manuscript; available in PMC: 2013 May 23.
Published in final edited form as: Science. 2012 Oct 18;338(6110):1069–1072. doi: 10.1126/science.1224409

Fig. 2.

Fig. 2

SAICAR binds to PKM2 in glucose-depleted cancer cells. (A) Cellular concentration of SAICAR in HeLa cells in response to glucose-depletion. HeLa cells were incubated in glucose-free media, and the time course changes in SAICAR concentration were measured using LC-MS as described in SOM. Pyruvate and lactate concentration changes are also noted. (B) Effect of extracellular glucose concentration on cellular SAICAR amount. Cellular concentration of SAICAR was measured in (○) HeLa and in (▽) NIH 3T3 cells 30 min after incubating in media with various glucose concentrations. (C) Effect of cell type. HeLa, H1299, HuMEC, and HuALF were incubated in 25 mM (filled box) or in no glucose media (gray box) for 30 min and used for the analysis of SAICAR level. Avg.±s.d. (n = 3). Other cancer cell lines tested (A549 and U87) also showed glucose-depletion dependent increase of cellular SAICAR level. (D) LC-MS peak area of SAICAR co-purified with PKM1, PKM2, or StrepII tag from H1299 cells incubated for 30 min in fresh 25 mM or no glucose media. (E) Amount of FBP that co-purified with the protein of interest analyzed as in (D).