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. Author manuscript; available in PMC: 2013 May 23.
Published in final edited form as: Science. 2012 Oct 18;338(6110):1069–1072. doi: 10.1126/science.1224409

Fig. 4.

Fig. 4

SAICAR-PKM2 interaction is responsible for the promotion of cancer cell survival. (A and B) Identification of a PKM2 mutant insensitive to SAICAR. Effect of (A) SAICAR and (B) FBP on pyruvate kinase activities of PKM2 Q393K (○) and PKM2 K433E (●) mutants are shown. Avg ± s.d. (n = 3). (C and D) Cell viability in glucose-depletion conditions was measured as in figure 3H in HeLa cells expressing (C) PKM2* and (D) PKM2* Q393K mutant while endogenous PKM2 expression is knocked down. Avg±s.d. (n = 3, >50 cells were counted for each measurement). Control: cells transfected with scrambled shRNA vectors.