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. Author manuscript; available in PMC: 2012 Dec 20.
Published in final edited form as: Endocr Relat Cancer. 2011 Dec 1;18(6):759–771. doi: 10.1530/ERC-10-0224

Figure 2. Effects of PKCδ knockdown by PKCδ-specific shRNA lentivirus on proliferation of human neuroendocrine tumor cells.

Figure 2

BON1 (A), CNDT 2.5 (B) and H727 (C) cells were grown to 50% confluence in 96-well plates and then infected with PKCδ-shRNA-Lentivirus (PKCδ-shRNA) or scrambled shRNA Lentivirus (sc-shRNA). Cell exposed to mock lentiviral infection (Vehicle) also served as controls. After 24, 48, and 72 hours of treatment, cell proliferation was evaluated by MTS assay. Control values were normalized to 100%. Error bars represent SEM. P values for comparison between control (scrambled shRNA) lentivirus and PKCδ-shRNA lentivirus effects on cell number reached significance at 24 hr of exposure (p ≤ 0.001) for all cell lines, and remained significant at the 48 and 72 hr time points.