Skip to main content
. Author manuscript; available in PMC: 2012 Dec 20.
Published in final edited form as: Endocr Relat Cancer. 2011 Dec 1;18(6):759–771. doi: 10.1530/ERC-10-0224

Figure 3. Cytotoxic effects of PKCδ-knockdown by shRNA-lentivirus on human neuroendocrine tumor cell lines.

Figure 3

BON1 (A), CNDT 2.5 (B) and H727 (C) cells were grown to 50% confluence in 96-well plates and then infected with PKCδ-shRNA-lentivirus or scrambled shRNA lentivirus (Vector). Cell exposed to mock lentiviral infection (Vehicle) also served as controls. After 24, 48 and 72 hr of treatment, cell cytotoxicity was evaluated by LDH-release assay. Total maximal LDH release was assigned the arbitrary value of 100%. Error bars represent SEM. P values for comparison between control (scrambled shRNA) lentivirus and PKCδ-shRNA lentivirus effects on LDH release reached significance at 24 hr of exposure (p ≤ 0.004) for all cell lines, and remained significant at the 48 and 72 hr time points.