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. 2012 Sep 24;12:67. doi: 10.1186/1472-6750-12-67

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Copyright ©2012 Ihssen et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Screening of OST mutant libraries. Sorted ELISA read-outs of 96-well random mutant libraries of PglB_Cj. Open circles: epPCR mutagenesis of the periplasmic domain (H423-I713), inactive clones selected for anti-HA screening and sequencing marked by an elipsoid. Open triangles: Cassette mutagenesis of I571 and V575 using primers with NDT degeneracy, active clones selected for sequencing marked by an elipsoid. Absorbance at 450 nm was measured against air. Filled symbols: wild-type controls on same plate (pGVXN112Kan), shaded symbols: inactive (mut) controls on same plate (pGVXN113Kan), host strain: E. coli CLM24 (pGVXN150-pGVXN345).