Table 2.
Expression and secretome data of predicted glycosyl hydrolases
| |
|
|
|
|
Transcriptomic data |
|
|
|
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| |
|
|
|
|
Expressiona |
|
Fold changesb |
|
FDR q-valuesb |
|
Secretome datac |
||||||||||
| Identifier | Gene | CAZy | (Predicted) function | SPd | Expe | Day 1 | Day 3 | Day 6 | Day 1 | Day 3 | Day 6 | Day 1 | Day 3 | Day 6 | Expe | Day 1 | Day 3 | Day 6 | |||
|
Chitin | |||||||||||||||||||||
| An09g02240 |
nagA |
GH20 |
β-1,6-N-acetylglucosaminidase |
1 |
1.1 |
64 |
52 |
48 |
|
57.4 |
46.4 |
43.5 |
|
2.4E-12 |
5.0E-12 |
5.6E-12 |
|
- |
++++ |
+++++ |
+++++ |
| An02g07020 |
chiB |
GH18 |
chitinase |
0 |
1.6 |
73 |
77 |
84 |
|
45.0 |
47.2 |
51.5 |
|
3.9E-14 |
4.1E-14 |
3.4E-14 |
|
- |
++ |
- |
- |
| An01g05360 |
cfcD |
GH18 |
chitinase |
0 |
1.2 |
10 |
11 |
10 |
|
8.1 |
8.9 |
7.7 |
|
2.8E-12 |
1.9E-12 |
3.7E-12 |
|
- |
- |
- |
- |
| An01g01920 |
|
GH20 |
β-1,6-N-acetylglucosaminidase |
1 |
0.5 |
2 |
1 |
2 |
|
3.1 |
2.5 |
3.6 |
|
5.6E-08 |
9.3E-07 |
1.5E-08 |
|
- |
- |
+++ |
++ |
| An02g02340 |
csmB |
GT2 |
chitin synthase |
0 |
7.7 |
19 |
25 |
22 |
|
2.5 |
3.2 |
2.9 |
|
1.8E-09 |
1.1E-10 |
2.9E-10 |
|
- |
- |
- |
- |
| An09g02290 |
chsD |
GT2 |
chitin synthase |
0 |
4.4 |
10 |
11 |
10 |
|
2.3 |
2.6 |
2.3 |
|
5.1E-09 |
1.4E-09 |
8.0E-09 |
|
- |
- |
- |
- |
| An09g04010 |
chsB |
GT2 |
chitin synthase |
0 |
7.5 |
16 |
16 |
17 |
|
2.1 |
2.1 |
2.2 |
|
4.1E-08 |
4.7E-08 |
1.9E-08 |
|
- |
- |
- |
- |
| An08g05290 |
chsG |
GT2 |
chitin synthase |
1 |
0.5 |
1 |
1 |
0 |
|
1.6 |
1.3 |
1.0 |
|
1.6E-04 |
1.5E-02 |
7.5E-01 |
|
- |
- |
- |
- |
| An02g02360 |
csmA |
GT2 |
chitin synthase |
0 |
2.9 |
5 |
6 |
4 |
|
1.6 |
2.0 |
1.5 |
|
2.1E-03 |
4.9E-05 |
6.5E-03 |
|
- |
- |
- |
- |
| An02g13580 |
cfcI |
GH18 |
chitinase |
1 |
0.6 |
1 |
24 |
43 |
|
2.3 |
41.6 |
73.5 |
|
1.2E-02 |
3.9E-09 |
6.9E-10 |
|
- |
- |
- |
- |
| An09g05920 |
ctcB |
GH18 |
chitinase |
1 |
0.5 |
1 |
16 |
42 |
|
1.3 |
33.8 |
90.2 |
|
1.2E-01 |
2.1E-11 |
1.2E-12 |
|
- |
- |
- |
+++∗ |
|
α-glucan |
|||||||||||||||||||||
| An07g08640 |
agnB |
GH71 |
α-glucanase |
1 |
0.5 |
33 |
27 |
6 |
|
68.7 |
57.7 |
13.4 |
|
8.4E-15 |
1.3E-14 |
1.2E-12 |
|
- |
+++ |
++ |
- |
| An15g04760 |
agnE |
GH71 |
α-glucanase |
1 |
0.4 |
0 |
2 |
1 |
|
1.0 |
3.6 |
1.7 |
|
7.2E-01 |
1.5E-08 |
1.6E-04 |
|
- |
- |
- |
- |
| An09g03100 |
agtA |
GH13 |
α-glucan transferase |
1 |
6.2 |
14 |
15 |
18 |
|
2.3 |
2.4 |
2.8 |
|
3.2E-04 |
1.9E-04 |
4.0E-05 |
|
- |
- |
- |
- |
| An02g03260 |
agsD |
GH13/GT5 |
α-glucan synthase |
1 |
0.4 |
0 |
1 |
1 |
|
1.0 |
2.3 |
1.5 |
|
9.5E-01 |
1.9E-06 |
1.4E-03 |
|
- |
- |
- |
- |
|
β-glucan | |||||||||||||||||||||
| An01g03090 |
|
GH81 |
β-glucanase |
1 |
1.1 |
34 |
56 |
57 |
|
29.8 |
49.3 |
50.5 |
|
1.1E-14 |
4.3E-15 |
3.7E-15 |
|
- |
- |
- |
- |
| An02g13180 |
|
GH55 |
β-glucanase |
1 |
0.4 |
4 |
2 |
3 |
|
11.1 |
6.1 |
8.1 |
|
5.4E-11 |
1.7E-09 |
2.9E-10 |
|
- |
+++ |
+++∗ |
++∗ |
| An01g04560 |
|
GH16 |
β-glucanase |
1 |
1.2 |
10 |
23 |
31 |
|
8.2 |
19.8 |
26.5 |
|
1.4E-11 |
3.3E-13 |
1.1E-13 |
|
- |
+++∗ |
++++ |
++++∗ |
| An18g04100 |
|
GH28 |
β-glucanase |
1 |
0.8 |
4 |
16 |
17 |
|
5.9 |
21.0 |
22.8 |
|
1.3E-07 |
2.5E-10 |
1.8E-10 |
|
- |
- |
- |
- |
| An01g11010 |
crhD |
GH16 |
β-glucanase |
1 |
21.3 |
92 |
61 |
44 |
|
4.3 |
2.9 |
2.1 |
|
1.2E-09 |
6.3E-08 |
4.6E-06 |
|
+++∗ |
+++++ |
+++++ |
+++++ |
| An07g04650 |
bgtC |
GH17 |
β-glucanase |
0 |
1.4 |
6 |
7 |
6 |
|
4.0 |
5.0 |
4.3 |
|
2.6E-09 |
5.3E-10 |
1.6E-09 |
|
+∗ |
- |
- |
- |
| An01g12450 |
bxgA |
GH55 |
β-glucanase |
1 |
7.5 |
26 |
23 |
34 |
|
3.5 |
3.0 |
4.5 |
|
6.4E-08 |
2.9E-07 |
7.6E-09 |
|
++∗ |
++++ |
+++++ |
+++++ |
| An11g01540 |
|
GH16 |
β-glucanase |
1 |
1.0 |
2 |
1 |
1 |
|
2.1 |
1.0 |
0.6 |
|
6.2E-06 |
9.9E-01 |
2.5E-04 |
|
++∗ |
++++ |
++++ |
++++∗ |
| An10g00400 |
gelA |
GH72 |
β-1,3-glucosyl transferase |
1 |
12.6 |
20 |
52 |
50 |
|
1.6 |
4.1 |
4.0 |
|
4.3E-05 |
1.6E-10 |
2.1E-10 |
|
+++∗ |
++ |
- |
- |
| An09g00670 |
gelD |
GH72 |
β-1,3-glucosyl transferase |
1 |
48.0 |
75 |
59 |
69 |
|
1.6 |
1.2 |
1.4 |
|
3.3E-05 |
1.5E-02 |
2.2E-04 |
|
+++ |
++++∗ |
+++++∗ |
+++++ |
| An02g00850 |
|
GH16 |
β-glucanase |
1 |
1.5 |
2 |
6 |
9 |
|
1.4 |
3.8 |
6.1 |
|
1.9E-03 |
1.3E-09 |
3.4E-11 |
|
- |
- |
- |
++ |
| An06g01550 |
fksA |
GT48 |
β-1,3-glucan synthase |
0 |
62.6 |
71 |
78 |
91 |
|
1.1 |
1.2 |
1.4 |
|
7.0E-02 |
2.6E-03 |
1.7E-05 |
|
- |
- |
- |
- |
| An08g03580 |
bgtA |
GH17 |
β-glucanase |
1 |
0.7 |
1 |
1 |
2 |
|
1.1 |
1.9 |
2.8 |
|
4.3E-01 |
1.5E-05 |
9.9E-08 |
|
- |
- |
- |
- |
| An02g09050 |
gelG |
GH72 |
β-1,3-glucosyl transferase |
1 |
0.6 |
1 |
2 |
6 |
|
1.0 |
3.6 |
9.0 |
|
9.3E-01 |
2.5E-06 |
4.6E-09 |
|
- |
- |
- |
- |
| An16g02850 |
|
GH16 |
β-glucanase |
1 |
1.4 |
1 |
5 |
3 |
|
0.8 |
3.6 |
2.3 |
|
1.3E-01 |
4.8E-09 |
7.7E-07 |
|
- |
- |
- |
- |
| An06g01530 |
|
GH17 |
β-glucanase |
1 |
0.6 |
1 |
1 |
3 |
|
0.8 |
2.3 |
4.4 |
|
2.5E-02 |
1.9E-07 |
2.3E-10 |
|
- |
- |
- |
- |
| An02g03980 |
kslA |
GH16 |
β-glucanase |
0 |
0.5 |
0 |
1 |
2 |
|
0.8 |
2.4 |
4.7 |
|
4.2E-03 |
1.5E-08 |
2.0E-11 |
|
- |
- |
- |
- |
|
Mannan | |||||||||||||||||||||
| An07g07700 |
|
GH76 |
α-1,6-mannanase |
1 |
1.4 |
30 |
35 |
27 |
|
20.8 |
24.7 |
18.8 |
|
7.1E-13 |
4.5E-13 |
1.1E-12 |
|
- |
+++ |
- |
- |
| An01g06500 |
dfgD |
GH76 |
α-1,6-mannanase |
1 |
0.5 |
3 |
5 |
6 |
|
5.8 |
8.4 |
10.9 |
|
2.3E-10 |
2.9E-11 |
7.0E-12 |
|
- |
- |
- |
- |
| An14g03520 |
dfgC |
GH76 |
α-1,6-mannanase |
1 |
3.6 |
7 |
6 |
7 |
|
1.9 |
1.8 |
1.9 |
|
1.1E-07 |
5.8E-07 |
1.9E-07 |
|
- |
++∗ |
+++∗ |
- |
| An04g09650 |
|
GH76 |
α-1,6-mannanase |
1 |
0.3 |
1 |
0 |
0 |
|
1.8 |
1.3 |
1.3 |
|
4.0E-05 |
3.4E-02 |
2.3E-02 |
|
- |
+++∗ |
+++∗ |
+++ |
| An02g02660 |
dfgG |
GH76 |
α-1,6-mannanase |
1 |
0.8 |
1 |
1 |
1 |
|
1.6 |
1.4 |
1.1 |
|
4.7E-03 |
3.5E-02 |
4.7E-01 |
|
- |
- |
- |
- |
| An18g01410 | dfgA | GH76 | α-1,6-mannanase | 1 | 0.7 | 1 | 1 | 2 | 0.8 | 1.5 | 2.7 | 2.4E-01 | 4.1E-03 | 1.9E-06 | - | - | - | - | |||
amRNA abundance relative (%) to the gamma-actin (An15g00560) encoding transcript during exponential growth.
bFold changes and FDR q-values for comparisons with transcriptome data from the exponential growth phas.
cProtein abundance in filtrates: (-) not detected; (+) < 5 ng ml−1; (++) < 50 ng ml−1; (+++) < 250 ng ml−1; (++++) < 1 μg ml−1; (+++++) < 4 μg ml−1; (++++++) > 4 μg ml−1; (∗) biological and/or technical relative standard deviation above 100 and 50, respectively.
dSignal peptide sequence prediction [43].
eExponential growth phase.