Table 5.
miRNAs putatively targeting flavonoid biosynthetic pathway
| miRNA | Sequence (5'-3') | V | M | S | H | Target (UniGene) | Score | Annotation |
|---|---|---|---|---|---|---|---|---|
| miR171 |
UUUUUCUGAUUGAGCCGUGCC |
17 |
51 |
19 |
6 |
S_CL1522Contig1 |
5.5 |
Anthocyanin synthase (ANS) |
| miR858b |
UUCGUUGUCUGUUCGACCUGA |
39 |
39 |
19 |
225 |
M_CL545Contig1 |
4 |
HTH_MYB |
| miR535 |
UGACGAUGAGAGAGAGCACGC |
67,950 |
30,364 |
24,750 |
51,647 |
S_CL160Contig1 |
6 |
Flavone synthase (FNS) |
| miR166i |
UGAAUGUCGUCUGGUUCGAAA |
106 |
80 |
68 |
51 |
S_CL5764Contig1 |
6 |
Flavonol synthase (FLS) |
| miR477b |
ACUCUCCCUCAAGAGCUUCUAG |
12 |
50 |
154 |
213 |
H_CL379Contig1 |
6 |
Dihydroflavonol-4-reductase (DFR) |
| miR159e |
UUUGGCUUGAAGGGAGCUCUA |
78 |
80 |
53 |
51 |
V_F1XUE2F01CHQVJ |
6 |
Cytochrome P450 |
| |
|
|
|
|
|
S_CL2652Contig1 |
6 |
Cytochrome P450 |
| miR172c |
GUAGCAUCAUCAAGAUUCAC |
7 |
4 |
2 |
58 |
H_CL3307Contig1 |
6 |
Cytochrome P450 |
| S_CL373Contig2 |
6 |
Cinnamate 4-hydroxylase (C4H) |
||||||
| |
|
|
|
|
|
V_CL1167Contig1 |
6 |
Cinnamate 4-hydroxylase (C4H) |
| miR399 |
GGGCGUCUUUCCUUUGGCAGG |
16 |
0 |
1 |
42 |
S_CL3909Contig1 |
6 |
Cytochrome P450 |
| miR396a |
CACAGCUUUCUUGAACUU |
11 |
22 |
43 |
7 |
H_CL735Contig1 |
5.5 |
Chalcone and stilbene synthase |
| H_F09TDQC01AFYKY |
5.5 |
Chalcone synthase (CHS) |
||||||
| M_FJZMSUQ02GLZSQ |
6 |
Short chain alcohol dehydrogenase |
||||||
| S_CL831Contig1 |
5.5 |
Chalcone and stilbene synthase |
||||||
| V_CL2603Contig1 |
5.5 |
Constitutive photomorphogenic DWARF (CPD) |
||||||
| V_CL3087Contig1 |
5 |
Cytochrome P450 |
||||||
| |
|
|
|
|
|
V_F1XUE2F01B3HEY |
5 |
Cinnamate 4-hydroxylase (C4H) |
| miR845e |
ACCUGGCUCUGAUACCAAUUG |
84 |
387 |
63 |
947 |
H_CL735Contig1 |
5.5 |
Chalcone and stilbene synthase family protein |
| |
|
|
|
|
|
S_CL831Contig1 |
5.5 |
Chalcone and stilbene synthase family protein |
| miR396e |
UUCCACAGCUUUCUUGAACUU |
6,309 |
13,690 |
7,655 |
2,520 |
H_CL11974Contig1 |
6 |
Cytochrome P450 |
| miR319 |
UGGACUGAAGGGAGCUCCC |
7 |
9 |
2 |
151 |
M_FJZMSUQ02HT34C |
4.5 |
Cytochrome P450 |
| miR396b |
CACAGCUUUCUUGAACUG |
20 |
39 |
62 |
22 |
S_FJZMSUQ02HBX1V |
6 |
Cytochrome P450 |
| V_CL10716Contig1 |
6 |
CPD (CONSTITUTIVE PHOTOMORPHOGENIC DWARF) |
||||||
| V_CL1787Contig1 |
6 |
Flavone synthase (FNS) |
||||||
| V_CL2603Contig1 |
6 |
CPD (CONSTITUTIVE PHOTOMORPHOGENIC DWARF) |
||||||
| V_F1XUE2F01B3HEY | 6 | C4H (CINNAMATE-4-HYDROXYLASE) |
The bold characters denote enriched miRNA tags with less than p<0.001 by analyzing Audic’s test [88]. Abbreviations: V: ‘Vital’; M: ‘Marcia’; S: ‘Sympathy’; H: ‘Haedang’.